Product Name:
GIT1-pY545
Product Number:
ab-pn517
Target Full Name: ARF GTPase-activating protein GIT1
Target Alias: ARF GAP GIT1; ARF GTPase-activating protein GIT1; CAT-1; CAT1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GIT1; GRK-interacting protein 1
Product Type Specific: GTPase-activating protein phosphosite-specific antibody
Antibody Code: PN517
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y545
Protein UniProt: Q9Y2X7
Protein SigNET: Q9Y2X7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GIT1 sequence peptide Cat. No.: PE-04AEN99
Antibody Immunogen Sequence: DAI(pY)SVH(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D542 to H548; In the last third of the protein before the GIT1_C domain. This is the major in vivo phosphorylation site in GIT1.
Production Method: Corresponds to amino acid residues D542 to H548; In the last third of the protein before the GIT1_C domain. This is the major in vivo phosphorylation site in GIT1.
Antibody Modification: GTPase-activating protein phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-95 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 and Jurkat cells.
Related Product 1: GIT1-pY545 blocking peptide
Related Product 2: GIT1 pan-specific antibody (Cat. No.: AB-NN261-1)
Scientific Background: GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signalling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. It increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
