Product Name:
Gab1-pY406
Product Number:
ab-pn516
Target Full Name: GRB2-associated-binding protein 1
Target Alias: GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Product Type Specific: Gab1 phosphosite-specific antibody
Antibody Code: PN516
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y406
Protein UniProt: Q13480
Protein SigNET: Gab1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Gab1 sequence peptide Cat. No.: PE-04ACW80
Antibody Immunogen Sequence: QDC(pY)DIP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q403 to P409; in the mid-region of the protein. The effect of Y406 phosphorylation is unclear. This is the second major in vivo phosphorylation site of Gab1 (≥1095 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Gab1 is known to be phosphorylated at this site in vitro by EGFR, and Met.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-strong immunoreactivity of a target-sized protein by Western blotting in HeLa cells (harvested in SDS-PAGE sample buffer).
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse | Platypus | Chicken | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 75-100 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactivities detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells, and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE buffer.
Related Product 1: Gab1 (403-409) pY406 antibody blocking peptide (Cat. No.: PE-04ACW80)
Scientific Background: Gab1 is an adapter protein that plays a role in intracellular signalling cascades triggered by activated receptor-type kinases. It plays a role in FGFR1 signalling and is probably involved in signalling from the epidermal growth factor receptor (EGFR), the insulin receptor (INSR), the hepatocyte growth factor receptor (Met), and the platelet-derived growth factor receptor (PDGFR) to pathways controlling cell growth, survival, and migration. It features an N-terminal pleckstrin homology (PH) domain, which binds to phosphatidylinositol 3,4,5-trisphosphate (PIP3) for localization to the plasma membrane, and multiple tyrosine phosphorylation sites that recruit signaling proteins. These include various SH2-domain-containing effector proteins (e.g., PI3K, SHP2, CrkL, PLCg) that bind to these phosphotyrosine residues. It is important for embryogenesis, with deficiency resulting in developmental defects in mouse models. GAB1 plays a significant role in tumorigenesis. High Gab1 expression levels are associated with poor prognosis and increased cancer proliferation (e.g., breast and lung, migration, and anti-apoptotic behaviour. This description may include information annotated by UniProt and/or Google AI.