Product Name:
ESYT1-pY822
Product Number:
ab-pn514
Target Full Name: Extended synaptotagmin-1
Target Alias: E-Syt1; ESYT1; Extended synaptotagmin-1; extended synaptotagmin-like protein 1; FAM62A; family with sequence similarity 62 (C2 domain containing), member A; KIAA0747; MBC2; Membrane-bound C2 domain-containing protein
Product Type Specific: ESYT1 phosphosite-specific antibody
Antibody Code: PN514
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y822
Protein UniProt: Q9BSJ8
Protein SigNET: ESYT1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ESYT1 sequence peptide Cat. No.: PE-04AHJ99
Antibody Immunogen Sequence: LSP(pY)ATL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L819 to L825; in the fourth C2 domain. The effect of Y822 phosphorylation is unclear. This is the major in vivo phosphorylation site in ESYT1 (≥1233 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in HepG2 and A431 cells of a ~122 kDa protein, but a slightlyStronger 140 kDa immunoreactive protein might be the target.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-150 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2 and A431 cells, but a 75 kDa medium cross-reactive protein can be observed in both celll lnes, and a ~50 kDa medium cross-reactive protein in A431cells.
Related Product 1: ESYT1 (819-825) pY822 antibody blocking peptide (Cat. No.: PE-04AHJ99)
Scientific Background: ESYT1 (Extended Synaptotagmin-1) is an endoplasmic reticulum (ER)-anchored protein that binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport. It is anchored to the ER via an N-terminal hairpin, with a SMP (synaptotagmin-like, mitochondrial, and lipid-binding) domain and multiple C2 domains that bind phospholipids and calcium. It translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. It helps to tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane. It has also been found to localize at ER-mitochondria contact sites, and plays a role in regulating mitochondrial lipid transport and respiration. It is widely expressed across various tissues, including adipocytes and smooth muscle cells. This description may include information annotated by UniProt and/or Google AI.