Product Name:
ErbB2IP-pY1104
Product Number:
ab-pn513
Target Full Name: Protein LAP2
Target Alias: Densin-180-like protein; erbb2 interacting protein; Erbb2-interacting protein; ErbB2IP; ERBIN; KIAA1225; LAP2; Protein LAP2
Product Type Specific: ERBB2IP phosphosite-specific antibody
Antibody Code: PN513
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1104
Protein UniProt: Q96RT1
Protein SigNET: ErbB2IP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ErbB2IP (Erbin) sequence peptide Cat. No.: PE-04AER99
Antibody Immunogen Sequence: EGD(pY)LSY(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E1101 to Y1107; in the C-terminal quarter of the protein before the PDZ domain. The effect of Y1104 phosphorylation is unclear. This is the major in vivo phosphorylation site in ERBB2IP (≥911 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-weak immunoreactivity of a target-sized protein by Western blotting in HEK-293 and HeLa cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Mouse | Platypus.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 170-180 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and HeLa cells, except for a very strong ~250 kDa cross-reactive protein in HeLa cells.
Related Product 1: ErbB2IP (1101-1107) pY1104 antibody blocking peptide (Cat. No.: PE-04AER99)
Scientific Background: ErbB2IP (ErbB2-interacting protein; Erbin) acts as a 180 kDa adapter for the receptor ErbB2, in epithelia. It belongs to the LAP (LRR and PDZ) family and functions primarily by regulating the localization of the ErbB2/HER2 receptor to the basolateral membrane of epithelial cells. ErbB2IP specifically binds to the unphosphorylated C-terminus of the ErbB2 receptor, restricting its presence to the lateral membrane. It features an N-terminal LRR domain (with 17 leucine-rich repeat)s and a C-terminal PDZ domain. By binding the unphosphorylated Tyr-1248 of receptor ErbB2, it may contribute to stabilize this unphosphorylated state. ErbB2IP plays a key role in cell polarity and signalling. It Inhibits NOD2-dependent NF-kappa-B signalling and proinflammatory cytokine secretion. It acts as a signaling scaffold, influencing the Ras-Raf interaction, TGF-beta signalling, and inhibiting invadopodia formation. It is found in the basolateral membrane, cytoplasm, and nuclear membrane, with high expression in the brain, heart, muscle, and digestive system. ErbB2IP has been identified as an autism-associated protein, involved in protein interaction modules related to neurological disorders. Overexpression of ERBB2IP has been implicated in tumour growth, specifically in non-small cell lung cancer and metastasis of colorectal cancer. This description may include information annotated by UniProt and/or Google AI.