Product Name:
ENO2-pY25
Product Number:
ab-pn512
Target Full Name: Gamma-enolase
Target Alias: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2; enolase 2 (gamma, neuronal); Gamma-enolase; Neural enolase; neuron specific gamma enolase; Neuron-specific enolase; neurone-specific enolase; NSE
Product Type Specific: Enolase phosphosite-specific antibody
Antibody Code: PN512
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y25
Protein UniProt: P09104
Protein SigNET: P09104
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ENO2 sequence peptide Cat. No.: PE-04ACT95
Antibody Immunogen Sequence: VDL(pY)TAK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V22 to K28; In the Enolase_N domain. This is one of the major in vivo phosphorylation sites in ENO2.
Production Method: Corresponds to amino acid residues V22 to K28; In the Enolase_N domain. This is one of the major in vivo phosphorylation sites in ENO2.
Antibody Modification: Enolase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-55 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactivities detected in A431 and A549 cells, although background is a little high with EGF treatment of A549 cells
Related Product 1: ENO2-pY25 blocking peptide
Related Product 2: ENO1-pY44 phosphosite-specific antibody (Cat. No.: AB-PN511)
Scientific Background: ENO2 (gamma-enolase) catalyzes the biochemical reaction: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O. This reaction is part of the metabolic pathway by which pyruvate is produced from D-glyceraldehyde 3 -phosphate. It also has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons.
