Product Name:
ENO1-pY44
Product Number:
ab-pn511
Target Full Name: Alpha-enolase
Target Alias: 2-phospho-D-glycerate hydro-lyase; alpha enolase like 1; Alpha-enolase; C-myc promoter-binding protein; ENO1; ENO1L1; ENOA; Enolase 1; enolase 1, (alpha); MBP-1; MBPB1; MPB-1; MPB1; MYC promoter-binding protein 1; NNE; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; PPH; tau-crystallin
Product Type Specific: Enolase phosphosite-specific antibody
Antibody Code: PN511
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y44
Protein UniProt: P06733
Protein SigNET: P06733
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ENO1 sequence peptide Cat. No.: PE-04ACS99
Antibody Immunogen Sequence: TGI(pY)EAL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T41 to L47; In the Enolase_N domain. This is the major in vivo phosphorylation site in ENO1.
Production Method: Corresponds to amino acid residues T41 to L47; In the Enolase_N domain. This is the major in vivo phosphorylation site in ENO1.
Antibody Modification: Enolase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-55 kDa.
Related Product 1: ENO1-pY44 blocking peptide
Related Product 2: ENO2-pY25 phosphosite-specific antibody (Cat. No.: AB-PN512)
Scientific Background: ENO1 (alpha-enolase) is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. As a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons, it also functions in the intravascular and pericellular fibrinolytic system. It may act as a tumour suppressor.