Product Name:
EML4-pY226
Product Number:
ab-pn510
Target Full Name: Echinoderm microtubule-associated protein-like 4
Target Alias: C2orf2; DKFZp686P18118; echinoderm microtubule associated protein like 4; Echinoderm microtubule-associated protein-like 4; ELP120; EMAL4; EMAP-4; EMAPL4; EML4; FLJ10942; FLJ32318; Restrictedly overexpressed proliferation-associated protein; Ropp 120; ROPP120
Product Type Specific: EML4 phosphosite-specific antibody
Antibody Code: PN510
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y226
Protein UniProt: Q9HC35
Protein SigNET: EML4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EML4 sequence peptide Cat. No.: PE-04AHG99
Antibody Immunogen Sequence: EGE(pY)IKM(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E223 to M229; in the N-terminal portion of EML4 near the HELP domain. The effect of Y226 phosphorylation is unclear. This is the major in vivo phosphorylation site in EML4 (≥408 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 115-125 kDa.
Related Product 1: EML4 (223-229) pY226 antibody blocking peptide (Cat. No.: PE-04AHG99)
Scientific Background: EML4 is a microtubule-binding protein that may modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic. It is essential for microtubule stability and helps organize the mitotic spindle during cell division. It is involved in proper kinetochore attachment to microtubules and promotes the recruitment of NUDC to the mitotic spindle. EML4 features a tandem atypical propeller domain (TAPE), a hydrophobic motif (HELP), a basic region, and a trimerization domain (TD). It is widely expressed in human tissues. The N-terminal region of EML4 fuses with the kinase domain of ALK (Anaplastic Lymphoma Kinase). The EML4 coiled-coil domain (part of the TD) facilitates constitutive dimerization of the fusion protein, leading to constant, oncogenic activation of ALK. The EML4-ALK fusion protein is a potent oncogene found in 4-5% of non-small cell lung cancers (NSCLC), often in young, non-smoking adenocarcinoma patients. EML4-ALK positive tumours often exhibit a specific, aggressive signet-ring cell pattern. This description may include information annotated by UniProt and/or Google AI.