Product Name:
EEF1A1-pY141
Product Number:
ab-pn509
Target Full Name: Eukaryotic elongation factor 1-alpha 1
Target Alias: CCS-3; CCS3; cervical cancer suppressor 3; CTCL tumour antigen; EEF-1; EEF1A; eEF1A-1; EEF1A1; EF-1-alpha-1; EF-Tu; EF1A; EF1a-like protein; EF1A1; elongation factor 1 alpha subunit; Elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1; eukaryotic translation elongation factor 1 alpha 1-like 14; FLJ25721; glucocorticoid receptor AF-1 specific elongation factor; GRAF-1EF; HNGC:16303; LENG7; leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; MGC102687; MGC131894; MGC16224; prostate tumour-inducing protein 1; PTI1; translation elongation factor 1 alpha 1-like 14
Product Type Specific: EEF1A1 phosphosite-specific antibody
Antibody Code: PN509
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y141
Protein UniProt: P68104
Protein SigNET: EEF1A1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EEF1A1 sequence peptide Cat. No.: PE-04ACQ90
Antibody Immunogen Sequence: LLA(pY)TLG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L138 to G144; in the middle of the GTP_EFTU domain. The effect of Y141 phosphorylation is unclear. This is the second major in vivo phosphorylation site in EEF1A1 (≥1206 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-50 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and Jurkat cells and sea star oocytes, except for a very strong ~75 kDa protein in FBS-treated Jurkat cells.
Related Product 1: EEF1A1 (138-144) pY141 antibody blocking peptide (Cat. No.: PE-04ACQ90)
Related Product 2: EEF2 T57 phosphorylation antibody (Cat. No.: AB-PN555)
Scientific Background: EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1) is an abundant translation protein that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Beyond translation, it acts as a multifunctional protein involved in heat shock response, actin bundling, and apoptosis regulation. It forms a complex with PARP1 and TXK that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. It is primarily found in the cytoplasm, with significant presence in the nucleus. It is widely expressed in tissues like the liver, lung, and pancreas. Its paralog, EEF1A2 (with 97% amino acid similarity), replaces it in adult brain, heart, and skeletal muscle. It is identified as an autoantigen in 66% of patients with Felty syndrome and is involved in cardiac disease through interaction with mineralocorticoid receptors. It is a mediator of lipotoxicity and is implicated in diseases like spinal and bulbar muscular atrophy (SBMA) and Amyotrophic Lateral Sclerosis (ALS) due to its role in the heat shock response. High expression of EEF1A1 is linked to tumor progression, metastasis, and poor prognosis in cancers like hepatocellular carcinoma and prostate cancer.This description may include information annotated by UniProt and/or Google AI.