Product Name:
EEF1A1-pY141
Product Number:
ab-pn509
Target Full Name: Eukaryotic elongation factor 1-alpha 1
Target Alias: CCS-3; CCS3; cervical cancer suppressor 3; CTCL tumour antigen; EEF-1; EEF1A; eEF1A-1; EEF1A1; EF-1-alpha-1; EF-Tu; EF1A; EF1a-like protein; EF1A1; elongation factor 1 alpha subunit; Elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1; eukaryotic translation elongation factor 1 alpha 1-like 14; FLJ25721; glucocorticoid receptor AF-1 specific elongation factor; GRAF-1EF; HNGC:16303; LENG7; leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; MGC102687; MGC131894; MGC16224; prostate tumour-inducing protein 1; PTI1; translation elongation factor 1 alpha 1-like 14
Product Type Specific: EEF1A1 phosphosite-specific antibody
Antibody Code: PN509
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y141
Protein UniProt: P68104
Protein SigNET: P68104
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EEF1A1 sequence peptide Cat. No.: PE-04ACQ90
Antibody Immunogen Sequence: LLA(pY)TLG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L138 to G144; In the middle of the GTP_EFTU domain. This is the second major in vivo phosphorylation site in EEF1A1.
Production Method: Corresponds to amino acid residues L138 to G144; In the middle of the GTP_EFTU domain. This is the second major in vivo phosphorylation site in EEF1A1.
Antibody Modification: EEF1A1 phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-50 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and Jurkat cells and sea star oocytes, except for a very strong ~75 kDa protein in FBS-treated Jurkat cells.
Related Product 1: EEF1A1-pY141 blocking peptide
Scientific Background: EEF1A1 promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It forms a comple with PARP1 and TXK that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
