Product Name:
Dok3-pY398
Product Number:
ab-pn508
Target Full Name: Docking protein 3
Target Alias: Dok-like protein; Dok3; DokL; Downstream of tyrosine kinase 3; FLJ22570; FLJ39939
Product Type Specific: Dok3 phosphosite-specific antibody
Antibody Code: PN508
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y398
Protein UniProt: Q7L591
Protein SigNET: Q7L591
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Dok3 sequence peptide Cat. No.: PE-04AHH99
Antibody Immunogen Sequence: EHL(pY)ENL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E395 to L401; In the last quarter of the protein. This is the major in vivo phosphorylation site in Dok3.
Production Method: Corresponds to amino acid residues E395 to L401; In the last quarter of the protein. This is the major in vivo phosphorylation site in Dok3.
Antibody Modification: Dok3 phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: At high concentrations, this antibody starts to detected multiple proteins in A431 cells.
Related Product 1: Dok3-pY398 blocking peptide
Scientific Background: Dok3 is an adaptor or scaffolding protein that is a negative regulator of JNK signalling in B-cells through interaction with INPP5D/SHIP1.
