Product Name:
Cas-L-pY166
Product Number:
ab-pn505
Target Full Name: Enhancer of filamentation 1
Target Alias: NEDD9; Neural precursor cell expressed developmentally down-regulated protein 9
Product Type Specific: Cas-L phosphosite-specific antibody
Antibody Code: PN505
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y166
Protein UniProt: Q14511
Protein SigNET: Q14511
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Cas-L sequence peptide Cat. No.: PE-04AFA99
Antibody Immunogen Sequence: GYV(pY)EYP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G163 to P169; In the region between the SH3_9 and Serine_rich domains. This is the second major in vivo phosphorylation site in Cas-L.
Production Method: Corresponds to amino acid residues G163 to P169; In the region between the SH3_9 and Serine_rich domains. This is the second major in vivo phosphorylation site in Cas-L.
Antibody Modification: Cas-L phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in HeLa cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Medium cross-reactive ~48 kDa protein detected in A431 and HeLa cells.
Related Product 1: Cas-L-pY166 blocking peptide
Scientific Background: CasL is a docking protein which plays a central coordinating role for tyrosine-kinase-based signalling related to cell adhesion. It functions in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. It may also participate in integrin beta-1 or B cell antigen receptor (BCR) mediated signalling in B- and T-cells.
