Product Name:
ANXA2-pY238
Product Number:
ab-pn504
Target Full Name: Annexin A2
Target Alias: Annexin II; Annexin-2; ANX2; ANX2L4; anxa 2; ANXA2; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; Chromobindin-8; LIP2; Lipocortin II; LPC2; LPC2D; p36; PAP-IV; Placental anticoagulant protein IV; Protein I
Product Type Specific: ANXA2 phosphosite-specific antibody
Antibody Code: PN504
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y238
Protein UniProt: P07355
Protein SigNET: ANXA2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ANXA2 sequence peptide Cat. No.: PE-04ACI99
Antibody Immunogen Sequence: YSP(pY)DML(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Y235 to L241; in the third Annexin domain. The effect of Y238 phosphorylation is unclear. This is the second major in vivo phosphoryation site in Annexin A2 with 964 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-40 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactive proteins detected in HeLa cells, Jurkat cells and in sea star oocytes.
Related Product 1: ANXA2 (235-241) pY238 antibody blocking peptide (Cat. No.: PE-04ACI99)
Related Product 2: ANXA1-pY207 phosphosite-specific antibody (Cat. No.: AB-PN503)
Related Product 3: ANXA11 Y482 phosphorylation antibody (Cat. No.: AB-PN871)
Scientific Background: Annexin A2 (ANXA2) is a calcium-regulated membrane-binding protein that binds two calcium ions with high affinity, which is greatly enhanced by anionic phospholipids. It may be involved in heat-stress response and inhibits PCSK9-enhanced LDL receptor degradation. It regulates membrane trafficking, endocytosis, exocytosis, and actin remodeling. It operates as a monomer in the cytoplasm or as a heterotetramer (with S100A10) on the cell surface to promote fibrinolysis, angiogenesis, and cell signaling. ANXA2 is predominantly located in the cytosol and on the cytoplasmic face of the plasma membrane, but can exist in the nucleus. It is upregulated in various cancers, including breast, liver, and prostate cancer, and facilitates tumour growth and metastasis. It is also implicated in neurodegenerative disorders like Alzheimer's and Parkinson’s. This description may include information annotated by UniProt and/or Google AI.