Product Name:
ANXA1-pY207
Product Number:
ab-pn503
Target Full Name: Annexin A1
Target Alias: Annexin A1; Annexin I; annexin I (lipocortin I); Annexin-1; ANX1; anxa 1; ANXA1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; LPC1; p35; Phospholipase A2 inhibitory protein
Product Type Specific: Annexin phosphosite-specific antibody
Antibody Code: PN503
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y207
Protein UniProt: P04083
Protein SigNET: P04083
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ANXA1 sequence peptide Cat. No.: PE-04AEY95
Antibody Immunogen Sequence: RAL(pY)EAG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R204 to G210; In the beginning of the third Annexin domain. This is the major in vivo phosphorylation site in ANXA1.
Production Method: Corresponds to amino acid residues R204 to G210; In the beginning of the third Annexin domain. This is the major in vivo phosphorylation site in ANXA1.
Antibody Modification: Annexin phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in HeLa cells, andMedium detection in A431 and MCF7 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 36-38 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Five other cross-reactive proteins in HeLa + phenylarsine oxide (PAO)+vanadate; 37 kDa target, 5 other cross-reactive proteins in A431 + EGF; 37 kDa target, 5 other cross-reactive proteins in MCF7 + Insulin.
Related Product 1: ANXA1-pY207 blocking peptide
Related Product 2: ANXA2-pY238 phosphosite-specific antibody (Cat. No.: AB-PN504)
Scientific Background: Annexin A1 plays important roles in the innate immune response as effector of glucocorticoid-mediated responses such as down-regulation of the early phase of the inflammatory response and as a regulator of the inflammatory process by promoting resolution of inflammation and wound healing. It functions at least in part by activating the formyl peptide receptors and downstream signalling.
