Product Name:
ACTN1-pY246
Product Number:
ab-pn502
Target Full Name: Alpha-actinin-1
Target Alias: actinin 1 smooth muscle; actinin, alpha 1; ACTN1; alpha-actinin 1; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1
Product Type Specific: Alpha-actinin phosphosite-specific antibody
Antibody Code: PN502
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y246
Protein UniProt: P12814
Protein SigNET: P12814
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ACTN1 sequence peptide Cat. No.: PE-04AIC99
Antibody Immunogen Sequence: SSF(pY)HAF(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S243 to F249; In the second CH domain. This is the major in vivo phosphorylation site in ACTN1.
Production Method: Corresponds to amino acid residues S243 to F249; In the second CH domain. This is the major in vivo phosphorylation site in ACTN1.
Antibody Modification: Alpha-actinin phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in FBS-treated A431 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Cross-reacts with a 85 kDa cross-reactive protein in A431 cells. This antibody cross-reacts with CDK1, ERK1 and at least 3 other proteins in seastar oocytes.
Related Product 1: ACTN1-pY246 blocking peptide
Scientific Background: ACTN1 is a bundling protein that cross-links F-actin to anchor actin to a variety of intracellular structures.
