Product Name:
ACTN1-pY246
Product Number:
ab-pn502
Target Full Name: Alpha-actinin-1
Target Alias: Actinin 1 smooth muscle; actinin, alpha 1; ACTN1; alpha-actinin 1; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1
Product Type Specific: ACTN1 phosphosite-specific antibody
Antibody Code: PN502
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y246
Protein UniProt: P12814
Protein SigNET: ACTN1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ACTN1 sequence peptide Cat. No.: PE-04AIC99
Antibody Immunogen Sequence: SSF(pY)HAF(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S243 to F249; in the second CH domain. The effect of Y246 phosphorylation is unclear. This is the major in vivo phosphorylation site in ACTN1 from mass spectrometry studies (≥528 reports in PhosphoSite Plus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in FBS-treated A431 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Fruit fly.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Cross-reacts with a 85 kDa cross-reactive protein in A431 cells. This antibody cross-reacts with CDK1, ERK1 and at least 3 other proteins in seastar oocytes.
Related Product 1: ACTN1 (243-249) pY246 antibody blocking peptide (Cat. No.: PE-04AIC99)
Scientific Background: Alpha-actinin-1 (ACTN1) is a non-muscle, actin-crosslinking protein crucial for organizing the cytoskeleton, anchoring actin filaments to the plasma membrane, and regulating cell motility, adhesion, and platelet formation. It acts as an antiparallel homodimer, forming bundles of filaments involved in cell structure and spreading. It is a 95-kDa, dumbbell-shaped protein containing an actin-binding domain and a Ca2+-dependent regulatory domain (EF-hands). It crosslinks actin filaments, specifically managing cortical actin reorganization in endothelial cells. It is located along microfilament bundles and in focal adhesions, facilitating cell motility, lamellipodial dynamics, and the organization of focal adhesions. Genetic variants in ACTN1 cause congenital macrothrombocytopenia, a condition characterized by low levels of abnormally large platelets. Increased expression of ACTNA has been linked to poor survival in osteosarcoma and enhanced cell invasion/migration in other cancers, making it a potential diagnostic marker. This description may include information annotated by UniProt and/or Google AI.