Product Name:
ACTB-pY53
Product Number:
ab-pn501
Target Full Name: Actin, cytoplasmic 1; Beta-actin
Target Alias: ACTB; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; PS1TP5-binding protein 1; PS1TP5BP1
Product Type Specific: ACTB phosphosite-specific antibody
Antibody Code: PN501
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y53
Protein UniProt: P60709
Protein SigNET: ACTB
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ACTB (Beta-actin) sequence peptide Cat. No.: PE-04AIB95
Antibody Immunogen Sequence: KDS(pY)VGD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K50 to D56; in the Actin domain. The effect of Y53 phosphorylation is unclear. This is one of the major in vivo phosphorylation sites in actin from mass spectrometry studies (≥1,087 reports recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Cow | Rat | Mouse | Chicken | Frog | Zebra fish | Tiger blowfish | Fruit fly | Honey bee | Nematode worm.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 42-45 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Prominent immunoreactivities in FBS-treated Jurkat cells at 75,to 120 kDa range were detected. No other cross-reactive proteins were observed in Jurkat, HEK-293 and seastar oocytes.
Related Product 1: ACTB (50-56) pY53 antibody blocking peptide (Cat. No.: PE-04AIB95)
Related Product 2: ACTB Y294 phosphorylation antibody (Cat. No.: AB-PN500)
Scientific Background: Actins are highly conserved proteins that are involved in various types of cell motility. They are a family of globular multi-functional proteins that form microfilaments in the cytoskeleton, and the thin filaments in muscle fibrils. It can be present as either a free monomer called G-actin (globular) or as part of a linear polymer microfilament called F-actin (filamentous), both of which are essential for such important cellular functions as the mobility and contraction of cells during cell division. It is found in essentially all eukaryotic cells, where it may be present at a concentration of over 100 μM. ACTB (Actin, cytoplasmic 1; beta-actin) is a 42 kDa protein that is highly conserved, and ubiquitously expressed, and essential for maintaining cell structure, integrity, and motility. Beyond the cytoplasm, beta-actin plays a key role in the nucleus, where it is involved in gene transcription, chromatin remodeling, and repair of damaged DNA. Beta-actin is required for early embryonic development and is critical for the migration and function of cells like neurons and white blood cells. Mutations in the ACTB gene disrupt the actin cytoskeleton, leading to various developmental disorders, including Baraitser-Winter Syndrome, which is a rare disorder affecting brain, eye, and facial development, commonly caused by R196H mutations. Dysregulation of beta-actin is also linked to cancer cell metastasis and immune cell dysfunction. This description may include information annotated by UniProt and/or Google AI.