Product Name:
ACTB-pY294
Product Number:
ab-pn500
Target Full Name: Actin, cytoplasmic 1
Target Alias: ACTB; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; PS1TP5-binding protein 1; PS1TP5BP1
Product Type Specific: Actin phosphosite-specific antibody
Antibody Code: PN500
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y294
Protein UniProt: P60709
Protein SigNET: P60709
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ACTB (Beta-actin) sequence peptide Cat. No.: PE-04AEV99
Antibody Immunogen Sequence: KDL(pY)ANT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K291 to T297; In the Actin domain. This is one of the major in vivo phosphorylation sites in actin.
Production Method: Corresponds to amino acid residues K291 to T297; In the Actin domain. This is one of the major in vivo phosphorylation sites in actin.
Antibody Modification: Actin phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium to weak immunoreactivity of a target-sized protein by Western blotting in HEK-293 and Jurkat cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 42-45 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Weak cross-reactivity with ~27 kDa protein in HEK-293 and Jurkat cells.
Related Product 1: ACTB-pY294 blocking peptide
Related Product 2: ACTB-pY53 phosphosite-specific antibody (Cat. No.: AB-PN501)
Scientific Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
