Product Name:
4E-BP1 pT45
Product Number:
ab-pn114
Target Full Name: Eukaryotic translation initiation factor 4E-binding protein 1 Thr-45 phosphosite
Target Alias: 4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein; P/OKCL.6; PHAS1; PHAS-1; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1
Product Type Specific: Phosphosite-specific polyclonal antibody for human 4E-BP1 T45 phosphosite
Antibody Code: PN114
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T45
Protein UniProt: Q13541
Protein SigNET: Q13541
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Peptide with amino acid sequence surrounding human 4E-BP1 Thr-45 phosphorylation site (Ser-Thr-pThr-Pro-Gly)
Antibody Immunogen Sequence: HSTpTPG
Antibody Immunogen Description: 6 amino acid phosphopeptide corresponding to the human 4E-BP1 T45 phosphosite
Production Method: 6 amino acid phosphopeptide corresponding to the human 4E-BP1 T45 phosphosite
Antibody Modification: Phosphosite-specific polyclonal antibody for human 4E-BP1 T45 phosphosite
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol
Storage Conditions: -20°C
Storage Stability: > 1 year
Product Use: Western blotting, Immunohistochemistry (IHC)
Antibody Dilution Recommended: 2 µg/mL
Antibody Potency: Very strong
Antibody Species Reactivity: Human, Mouse, Rat
Antibody Positive Control: T45 phosphorylated 4E-BP1
Antibody Specificity: Very high
Antibody Cross Reactivity: This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Ocassionally, strong cross-reactivities with ~90 kDa and ~160 kDa proteins can be detected.
Related Product 1: 4E-BP1-pT37+pT46 phosphosite-specific antibody (Cat. No.: AB-PN550)
Scientific Background: The protein synthesis inhibitor 4E-BP1 (PHAS1) normally competes with EIF4G1/EIF4G3 to interact with EIF4E to prevent its assembly into the EIF4F complex and thereby inhibits cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, and permits initiation of cap-dependent translation. 4E-BP1 phosphorylation is targeted for regulation by hormones, growth factors and other stimuli that signal through the PI3 kinase/mTORC1 and MAPK pathways. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1 and Ser-65 by ERK1/ERK2. It is also phosphorylated upon DNA damage, probably by ATM or ATR.
