Product Name:
4E-BP1 pT45
Product Number:
ab-pn114
Target Full Name: Eukaryotic translation initiation factor 4E-binding protein 1 Thr-45 phosphosite
Target Alias: 4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein; P/OKCL.6; PHAS1; PHAS-1; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1
Product Type Specific: 4E-BP1 phosphosite-specific antibody
Antibody Code: PN114
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T45
Protein UniProt: Q13541
Protein SigNET: 4E
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Peptide with amino acid sequence surrounding human 4E-BP1 Thr-45 phosphorylation site
Antibody Immunogen Sequence: FS(pT)TPG
Antibody Immunogen Description: 6 amino acid phosphopeptide corresponding to the human 4E-BP1 T45 phosphosite.
Production Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using the epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol
Storage Conditions: -20°C
Storage Stability: > 1 year
Product Use: Western blotting, Immunohistochemistry (IHC)
Antibody Dilution Recommended: 2 µg/mL
Antibody Potency: Very strong
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Zebra fish | Honey bee | Sea urchin.
Antibody Positive Control: T45 phosphorylated 4E-BP1
Antibody Specificity: Very high
Antibody Cross Reactivity: This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Ocassionally, strong cross-reactivities with ~90 kDa and ~160 kDa proteins can be detected.
Related Product 1: 4E-BP1 (33-40) pT37+pT46 antibody blocking peptide (Cat. No.: PE-04AQU90)
Related Product 2: 4E-BP1-pT37+pT46 phosphosite-specific antibody (Cat. No.: AB-PN550)
Scientific Background: 4E-BP1 (PHAS-1) acts downstream of the MAP kinase and TORC1 signalling pathways to inhibit EIF4E activity to promote translation initiation by preventing its assembly into the eIF4F complex. The hypophosphorylated form of 4E-BP1 competes with EIF4G1/EIF4G3 and strongly binds to EIF4E to repress translation. The hyperphosphorylated form of 4E-BP1 dissociates from EIF4E, and permits interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Phosphorylation of 4E-BP1 disrupts this binding, and permits initiation of cap-dependent translation. 4E-BP1 phosphorylation is targeted for regulation by hormones, growth factors and other stimuli that signal through the PI3 kinase/mTORC1 and MAPK pathways. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1 and Ser-65 by ERK1/ERK2. It is also phosphorylated upon DNA damage, probably by ATM or ATR. This description may include information annotated by UniProt and/or Google AI.