Product Name:
IQGAP2-pS1358
Product Number:
ab-pn1030
Target Full Name: GRB2-related adapter protein
Target Alias: IQ motif containing GTPase activating protein 2; IQGA2
Product Type Specific: IQGAP2 phosphosite-specific antibody
Antibody Code: PN1030
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S1358
Protein UniProt: Q13576
Protein SigNET: IQGAP2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human IQGAP2
Antibody Immunogen Sequence: HSQ(pS)MIEDAC
Antibody Immunogen Description: Corresponds to amino acid residues H1355 to A1363.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog.
Related Product 1: IQGAP1 S1443 phosphorylation antibody (Cat. No.: AB-PN1029)
Scientific Background: IQGAP2 (IQ motif-containing GTPase-activating protein 2) is a multidomain scaffolding protein that acts as a tumour suppressor and regulator of cell morphology, motility, and inflammatory responses. It binds F-actin, calmodulin, and Rho GTPases (Rac1/Cdc42; but not increasing their GTPase activity), acting primarily in the liver, where it counters oncogenic IQGAP1 and regulates metabolism, and suppresses liver tumorigenesis. It contains a calponin homology domain (CHD), WW domain, four IQ motifs (binding calmodulin), and a RasGAP-related domain. It is needed for the inflammatory response in the colon (via NF-κB/IL-6) and impacts blood-brain barrier integrity. Reduced expression of IQGAP2 is associated with severe liver, hepatocellular carcinoma, breast, gastric, and thyroid cancers, as well as Alzheimer’s disease. Loss of IQGAP2 in breast and gastric cancers promotes epithelial-mesenchymal transition (EMT) and cancer progression. Isoform 2 expression is enhanced in testis. This description may include information annotated by UniProt and/or Google AI.