Product Name:
GAP43-pS142+pT144
Product Number:
ab-pn1026
Target Full Name: Neuromodulin
Target Alias: Axonal membrane protein GAP-43; B-50; BASP2; Calmodulin-binding protein P-57; Growth associated protein 43; Growth-associated protein 43; Nerve growth-related peptide GAP43; NEUM; Neural phosphoprotein B-50; Neuron growth-associated protein 43; PP46; Protein F1
Product Type Specific: GAP43 phosphosite-specific antibody
Antibody Code: PN1026
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S142+T144
Protein UniProt: P17677
Protein SigNET: GAP43
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human GAP43
Antibody Immunogen Sequence: CAETE(pS)A(pT)KAS
Antibody Immunogen Description: Corresponds to amino acid residues A138 to S1477.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Frog.
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Scientific Background: GAP43 (Neuromodulin) belongs to the neuromodulin family, and is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons in developing and regenerating neurons. It acts as a key marker for nerve regeneration, promoting axon outgrowth, synaptic plasticity, and neurotransmitter release, while also playing a role in actin cytoskeleton regulation. It functions in axonal and dendritic filopodia induction. It contains an IQ domain, which binds to calmodulin, and is anchored to the membrane through palmitoylation. GAP43 is involved in axon regeneration, and its dysfunction has been linked to neurodevelopmental disorders and Alzheimer's disease. While primarily neuronal, it has been observed in some non-myelinating Schwann cells and some glial cells. This description may include information annotated by UniProt and/or Google AI.