Product Name:
MARCKS-pS27+pS29
Product Number:
ab-pn1023
Target Full Name: Myristoylated alanine-rich C-kinase substrate
Target Alias: 80K-L; 80K-L protein; MACS; MARCS; Myristoylated alanine-rich C-kinase substrate; Myristoylated alanine-rich protein kinase C substrate; PKCSL; PRKCSL; Protein kinase C substrate, 80 kDa protein, light chain
Product Type Specific: MARCKS phosphosite-specific antibody
Antibody Code: PN1023
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S27+S29
Protein UniProt: P29966
Protein SigNET: MARCKS
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human MARCKS
Antibody Immunogen Sequence: AAVAS(pS)P(pS)KANGC
Antibody Immunogen Description: Corresponds to amino acid residues A22 to G33.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Frog.
Scientific Background: MARCKS (Myristoylated Alanine-Rich C-Kinase Substrate) is an intrinsically disordered, membrane-associated protein that plays a role in the structural modulation of the actin cytoskeleton, chemotaxis, motility, cell adhesion, phagocytosis, and exocytosis (e.g., mucin secretion) through lipid sequestering and/or protein docking to membranes. It is widely expressed intracellular protein that acts as a major substrate for Protein Kinase C (PKC). It functions as a flexible, membrane-associated actin-organizing molecule that bridges the plasma membrane and the actin cytoskeleton, and regulates cell motility, adhesion, shape, and neurogenesis. MARCKS is involved in inflammation-driven immune cell migration and is often implicated in tumour progression. It is involved in phagocytosis, exocytosis (e.g., mucin secretion), and cell adhesion. It acts as a membrane anchor that can sequester phosphatidylinositol-4,5-diphosphate (PIP2) at lipid rafts in the plasma membrane of quiescent cells, an action reversed by protein kinase C, ultimately inhibiting exocytosis. It has a N-terminal myristoylated domain for membrane binding and a conserved phosphorylation site (Effector Domain). In its unphosphorylated state, MARCKS binds to the plasma membrane and cross-links actin filaments. When phosphorylated by PKC or bound by calcium/calmodulin, it translocates to the cytosol, releasing its hold on the membrane and actin. MARCKS is linked to cancer, specifically metastasis and tumour progression, and is involved in neurological disorders, including schizophrenia, bipolar disorder, and Alzheimer's disease. This description may include information annotated by UniProt and/or Google AI.