Product Name:
HOMER1-pS315
Product Number:
ab-pn1020
Target Full Name: Homer protein homolog 1
Target Alias: HOME1; Homer 1; HOMER1A; HOMER1B; HOMER-1B; HOMER1C; SYN47; Ves-1
Product Type Specific: HOMER1 phosphosite-specific antibody
Antibody Code: PN1020
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S315
Protein UniProt: Q86YM7
Protein SigNET: HOMER1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human HOMER1
Antibody Immunogen Sequence: RLEK(pS)QNEQ(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R311 to Q319.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.35 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog.
Related Product 1: HOMER3 S120 phosphorylation antibody (Cat. No.: AB-PN1017)
Scientific Background: HOMER1 (Homer protein homolog 1) is a postsynaptic density (PSD) scaffolding protein of the homer family that is critical for regulating excitatory synapses, glutamate receptor signaling, and calcium homeostasis. It plays a key role in neuronal plasticity, learning, and memory. It acts as an adaptor protein that binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. It may also couple GRM1 to PI3 kinase through its interaction with AGAP2. It regulates the maintenance of dendritic spines and the scaling of synaptic AMPA receptors. It occurs in different isoforms. Long-form proteins (Homer1b/c) are constitutive and scaffold synaptic proteins. The short-form, Homer1a, is an immediate-early gene product rapidly induced by high neuronal activity or during sleep deprivation that regulates synaptic strength by uncoupling mGluRs from their receptors, by acting as a dominant-negative regulator. It is involved in psychiatric disorders, including schizophrenia, autism, and depression, and mediates responses to psychoactive agents. It is predominantly expressed in the central nervous system, but also found in heart and skeletal muscle. This description may include information annotated by UniProt and/or Google AI.