Product Name:
HOMER3-pS120
Product Number:
ab-pn1017
Target Full Name: Homer protein homolog 3
Target Alias: Home3; homer 3; homer-3
Product Type Specific: HOMER3 phosphosite-specific antibody
Antibody Code: PN1017
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S120
Protein UniProt: Q9NSC5
Protein SigNET: HOMER3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human HOMER3
Antibody Immunogen Sequence: AREK(pS)QDGGC
Antibody Immunogen Description: Corresponds to amino acid residues A116 to G124.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rabbit | Rat | Mouse | Chicken | Frog .
Related Product 1: HOMER1 S315 phosphorylation antibody (Cat. No.: AB-PN1020)
Scientific Background: HOMER3 (Homer protein homolog 3) is a crucial, multifunctioning scaffolding protein of the Homer family that is primarily involved in bridging postsynaptic density proteins and regulating calcium signaling in neurons and immune cells. As part of the postsynaptic density (PSD), HOMER3 binds and cross-links the cytoplasmic domains of receptors like Group I metabotropic glutamate receptors (GRM1/5) and intracellular calcium channels (ITPR1, RYR1). It works with HOMER2 to inhibit the calcineurin-NFAT pathway, and act as a negative regulator of T-cell activation. It can act by competing with calcineurin/PPP3CA for NFAT protein binding, and blocking NFAT activation by PPP3CA. It contains an N-terminal EVH1 domain for protein interactions and a C-terminal coiled-coil domain for self-oligomerization. It is linked to neurological conditions such as Spinocerebellar Ataxia 1, is implicated in Acute Myeloid Leukemia (AML), and acts as a promoter of metastasis in triple-negative breast cancer (TNBC) by regulating beta-Catenin. It is highly expressed in the brain, but also found in other tissues like the liver and immune cells. This description may include information annotated by UniProt and/or Google AI.