Product Name:
CTMP-pS37
Product Number:
ab-pn1011
Target Full Name: Thioesterase superfamily member 4
Target Alias: Carboxyl-terminal modulator protein; C-terminal modulator protein; THEM4
Product Type Specific: CTMP (THEM4) phosphosite-specific antibody
Antibody Code: PN1011
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S38
Protein UniProt: Q5T1C6
Protein SigNET: CTMP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CTMP
Antibody Immunogen Sequence: LRSF(pS)SEEV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L32 to V41.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse.
Scientific Background: CTMP (Acyl-coenzyme A thioesterase; THEM4) is a cytosolic and mitochondrial enzyme that belongs to the THEM4/THEM5 thioesterase family. It exhibits acyl-CoA thioesterase activity towards medium and long-chain (C14 to C18) fatty acyl-CoA substrates, and probably plays a role in mitochondrial fatty acid metabolism. It catalyzes the reaction: hexadecanoyl-CoA + H2O = hexadecanoate + CoA + H+. It functions in the apoptotic process, possibly via its regulation of protein kinase B (PKB/Akt) activity and location. It binds to the regulatory domain of Akt and inhibits its phosphorylation, acting as a crucial modulator of cell survival, proliferation, and apoptosis. It is found predominantly in skeletal muscle, testis, uterus, brain and kidney. Its is down-regulated in glioblastoma or glioma compared to non-neoplastic brain due to promoter hypermethylation. This description may include information annotated by UniProt and/or Google AI.