Product Name:
eIF4G1-pT205+pT207
Product Number:
ab-pn1008
Target Full Name: Eukaryotic translation initiation factor 4 gamma 1
Target Alias: EIF4F; EIF4G; EIF-4G; EIF-4G 1; EIF-4G1; EIF-4-gamma 1; EIF4GI; Eukaryotic translation initiation factor 4 gamma, 1; Eukaryotic translation initiation factor 4GI; IF4G1; P220
Product Type Specific: eIF4G1 phosphosite-specific antibody
Antibody Code: PN1008
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T205+T207
Protein UniProt: Q04637
Protein SigNET: eIF4G1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human eIF4G1
Antibody Immunogen Sequence: CARTAS(pT)P(pT)PPQ
Antibody Immunogen Description: Corresponds to amino acid residues A200 to Q210. The effects of T205 and T207 phosphorylation are unknown. These are fairly major sites of in vivo phosphorylation with ≥46 and ≥47 mass spectrometry reports, respectively, in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Zebra fish.
Related Product 1: eIF2⍺ S52 phosphorylation antibody (Cat. No.: AB-PN820)
Related Product 2: EIF2AK1 S258 phosphorylation antibody (Cat. No.: AB-PK876)
Related Product 3: EIF2AK3 T982 phosphorylation antibody (Cat. No.: AB-PK604)
Related Product 4: EIF2AK3 S555 phosphorylation antibody (Cat. No.: AB-PK926)
Related Product 5: EIF2AK3 S715 phosphorylation antibody (Cat. No.: AB-PK927)
Related Product 6: EIF2AK4 T667 phosphorylation antibody (Cat. No.: AB-PK877)
Related Product 7: eIF4B S504 phosphorylation antibody (Cat. No.: AB-PN819)
Related Product 8: eIF4E S209 phosphorylation antibody (Cat. No.: AB-PN956)
Related Product 9: eIF4G1 S1185+S1187 phosphorylation antibody (Cat. No.: AB-PN1004)
Related Product 10: EEF2 T57 phosphorylation antibody (Cat. No.: AB-PN555)
Scientific Background: eIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma 1) is a critical, large scaffolding protein that belongs to the eukaryotic initiation factor 4G family. eIF4G1 is vital for cellular growth, brain development, and is often overexpressed in cancers. It is essential for cap-dependent mRNA translation initiation that acts as a key component of the eIF4F complex. It binds eIF4E (cap-binding), eIF4A (helicase), and eIF3 (ribosome), and facilitates ribosome recruitment. It features distinct binding domains for eIF4E (N-terminal), eIF3/eIF4A (central), and a second eIF4A-binding site in the C-terminal. It exists in two complexes, either with EIF1 or with EIF4E. Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon. Together with EIF4E, it antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning. It facilitates 5' UTR scanning, cap-dependent, and some cap-independent initiation processes, notably aiding translation during cellular stress. As a member of the eIF4F complex, it is required for endoplasmic reticulum stress-induced ATF4 mRNA translation. Deficiency of eIF4G1 is linked to impaired hippocampus-dependent learning, reduced mitochondrial energy production, and abnormal neuron morphology. It is overexpressed in various cancers (e.g., prostate, breast), driving tumour growth, survival, and invasion. This description may include information annotated by UniProt and/or Google AI.