Product Name:
VAMP4-pS30
Product Number:
ab-pn1002
Target Full Name: Vesicle-associated membrane protein 4
Target Alias: VAMP-4
Product Type Specific: VAMP4 phosphosite-specific antibody
Antibody Code: PN1002
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S30
Protein UniProt: O75379
Protein SigNET: VAMP4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human VAMP4
Antibody Immunogen Sequence: EDD(pS)DEE(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E27 to E33. The effect of S30 phosphorylation is unclear. This is the major in vivo site of phosphorylation of VAMP4 based on ≥84 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Platypus | Chicken .
Related Product 1: VAMP1/2 expression antibody (Cat. No.: AB-NN358-1)
Related Product 2: VAMP1 S63 phosphorylation antibody (Cat. No.: AB-PN1001)
Scientific Background: VAMP4 (Vesicle-associated membrane protein 4) is a SNARE protein belongs to the synaptobrevin family. It is primarily localized to the trans-Golgi network (TGN) and endosomes, crucial for intracellular membrane trafficking. As a v-SNARE, it mediates vesicle docking and fusion, specifically regulating retrograde transport from endosomes to the TGN and synaptic vesicle release. VAMP4 is essential for activity-dependent bulk endocytosis and maintains a pool of synaptic vesicles that support asynchronous neurotransmission. VAMP4 is involved in regulating secretory granule maturation by potentially eliminating inhibitors (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. It functions by forming SNARE complexes with partners such as syntaxin 6 (STX6), syntaxin 16 (STX16), and Vti1a. It is highly enriched in the trans-Golgi network (TGN), tubular-vesicular membranes, and immature secretory granules. It is involved in the TGN-to-endosome transport, retrograde transport from early endosomes to the TGN, and homotypic fusion of early endosomes. It features a C-terminal hydrophobic domain that acts as a membrane anchor. Depletion of VAMP4 causes fragmentation of the Golgi ribbon structure, indicating that it has a role in maintaining Golgi integrity. This description may include information annotated by UniProt and/or Google AI.