Product Name:
VAMP1-pS63
Product Number:
ab-pn1001
Target Full Name: Vesicle-associated membrane protein 1 Synaptobrevin-1
Target Alias: SYB1; Synaptobrevin; Synaptobrevin-1; vesicle associated membrane protein
Product Type Specific: VAMP1 phosphosite-specific antibody
Antibody Code: PN1001
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S63
Protein UniProt: P23763
Protein SigNET: VAMP1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human VAMP1
Antibody Immunogen Sequence: DQKL(pS)ELD(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues D59 to D66. The effect of S63 phosphorylation is unclear. This is the major in vivo site of phosphorylation of VAMP1 based on ≥22 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.7 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Nematode worm.
Related Product 1: VAMP1/2 expression antibody (Cat. No.: AB-NN358-1)
Related Product 2: VAMP4 S30 phosphorylation antibody (Cat. No.: AB-PN1002)
Scientific Background: VAMP1 (vesicle-associated membrane protein) is an 18 kDa member of the synaptobrevin family of proteins. It is an integral membrane protein crucial for neurotransmitter release and vesicle fusion at the presynaptic nerve terminal. It acts as a key component of the SNARE complex, mediating docking and exocytosis of synaptic vesicles. It participates in vesicle fusion with the plasma membrane (1). Synaptobrevins, Syntakins and the 25 kDa synaptosomal associated SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane (2). VAMP-1 is also one of the key proteins in the SNARE complex which is involved in regulated exocytosis (3). Primarily found in the nervous system (specifically brain and spinal cord) and neuromuscular junctions, with some isoforms expressed in other tissues (e.g., kidney, exocrine pancreas). Multiple splice variants exist, with VAMP1A (primarily neural) and VAMP1B (broadly expressed) being common. Mutations in VAMP1 are linked to serious disorders such as congenital myasthenic syndromes and hereditary spastic ataxia, resulting from weakened neurotransmitter release. VAMP1 is a target for botulinum neurotoxin serotype D, which cleaves the protein, causing neuromuscular paralysis. This description may include information annotated by UniProt and/or Google AI.