Product Name:
PKCq-pS662
Product Number:
ab-pk971
Target Full Name: Protein-serine kinase C theta
Target Alias: Kinase PKC-theta; KPCT; MGC126514; MGC141919; NPKC-theta; PKCQ; PKC-theta; PRKCQ; PRKCT; Protein kinase C, theta; Protein kinase C, theta type; CCDS7079.1; Q04759; ENSG00000065675
Product Type Specific: PKCt protein kinase phosphosite-specific antibody
Antibody Code: PK971
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S662
Protein UniProt: Q04759
Protein SigNET: PKCq
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PKCq
Antibody Immunogen Sequence: PFDS(pS)NFD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues P658 to D665.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.45 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus.
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Related Product 2: PKCA/BSubtide kinase substrate peptide (Cat. No.: PE-01BIR95)
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Related Product 7: PKCq S685 phosphorylation antibody (Cat. No.: AB-PK961)
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Scientific Background: PKCt (PKCq, PRKCT, PKC-theta) is a protein-serine/threonine kinase of the AGC group and novel PKC family. It is dependent on acidic phospholipids (e.g. phosphatidylserine) and diacylglycerol for full phosphotransferase activity, and does not require calcium. It is essential in TCR signalling for T-cell activation, differentiation, proliferation, and survival. These T-cell responses are mediated via Jun, NF-kappa-B, NFATC1 and NFATC2. The phosphotransferase activity of PKCt is unaffected by an Y90F mutation, but T-cell proliferation is inhibited. An A148E mutation results in constitutive activation of PKCt. A T219A mutation again will not inhibit kinase phosphotransferase activity, but it will inhibit localization to the plasma membrane and inhibit transactivation of IL2 promoter. Phosphorylation at Y90, T538 (activation loop of the kinase domain) and S676 (turn motif) increases phosphotransferase activity. Phosphorylation at S695 (hydrophobic region) increases phosphotransferase activity and interaction with PDK1. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. PKCt is linked to Gastrointestinal Stromal Tumours (GANT, GIST), which are rare sarcomas arising from tissues lining the gastrointestinal tract and typically arise from the stomach, intestines, or colon. GANT has been related to neurofibromatosis and adenocarcinoma as well. This description may include information annotated by UniProt and/or Google AI.