Product Name:
JNK1-pT183
Product Number:
ab-pk969
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinase (SAPK)) 1
Target Alias: c-Jun N-terminal kinase 1; JNK; JNK1A2; JNK1-alpha-2; JNK-46;JNK21B1/2; JUN N-terminal kinase; Kinase JNK1; MAPK8; MK08; PRKM8; SAPK1; SAPK1c; Stress-activated protein kinase JNK1; CCDS7225.1; ENSG00000107643
Product Type Specific: JNK1 (MAPK8) protein kinase phosphosite-specific antibody
Antibody Code: PK969
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T183
Protein UniProt: P45983
Protein SigNET: JNK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human JNK1
Antibody Immunogen Sequence: SFMM(pT)P(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S179 to P184.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Frog.
Related Product 1: JNKSelectideA kinase substrate peptide (Cat. No.: PE-14CAD90)
Related Product 2: JNKSubtide kinase substrate peptide (Cat. No.: PE-01BHT95)
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Related Product 5: JNK1 Y185 phosphorylation antibody (Cat. No.: AB-PK670)
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Scientific Background: JNK1 (MAPK8) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is involved in the regulation of several cellular processes, including cell proliferation, differentiation, migration, transformation, and apoptosis, through the JNK/SAP signalling pathway. This pathway is activated by extracellular stimuli such as pro-inflammatory cytokines or physical stress, as well as signals of intracellular stress, such as misfolded proteins in the endoplasmic reticulum. It is also activated by high fat diet in fat, muscle, and liver tissues, leading to increased phosphorylation of IRS1 and contributing to insulin resistance. Phosphorylation of Thr-183 and Tyr-185 by either of two dual specificity kinases, MAP2K4 and MAP2K7, increases phosphotransferase activity. It is inhibited by dual specificity phosphatases, such as DUSP1, which target the T183 and Y185 activatory phosphorylation sites. It promotes apoptosis (cell death) by upregulating pro-apoptotic genes, but can also promote survival in specific contexts. Apart from c-Jun, substrates include proteins involved in T-cell differentiation, microtubule dynamics (via STMN2 phosphorylation), and the circadian clock. JNK1 is associated with cancer pathogenesis, including liver and colorectal cancers, as well as obesity-induced inflammation. This description may include information annotated by UniProt and/or Google AI.