Product Name:
Akt1-pS473
Product Number:
ab-pk958
Target Full Name: RAC-alpha serine/threonine-protein kinase
Target Alias: Akt; Akt1; Akt1 kinase; C-Akt; Kinase Akt1; PKB; PKB-alpha; PRKBA; V-akt murine thymoma viral oncogene 1; Protein kinase B; RAC; RAC-alpha serine,threonine kinase; RAC-alpha serine/threonine kinase; RAC-PK-alpha; PKBA; hCG_96740; Akt1_NEW; Akt; MGC99656; CCDS9994; B0LPE5; B2RAM5; B3KVH4; B3KXD7; B7Z5R1; Q9BV07; ENSG00000142208
Product Type Specific: Akt1 protein kinase phosphosite-specific antibody
Antibody Code: PK958
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S473
Protein UniProt: P31749
Protein SigNET: Akt1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Akt1
Antibody Immunogen Sequence: FPQF(pS)YSAS(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues F469 to S477. S473 phosphorylation stimulates phosphotransferase activity of Akt1, and regulates cell cycle progression, and its intracellular location. This is the major site of phosphorylation of Akt1 identified by mass spectrometry studies (≥73 reports by high throughput and 1,960 reports by low throughput in PhosphoSitePlus) and over 1391 scientific publications involving primarily Western blotting. Akt1 is known to be phosphorylated in vitro at S473 by ATM, ATR, DNAPK, FRAP1, IKKE, ILK, LRRK2, MAPKAPK2, MAPKAPK5, mTOR, p38a MAPK, PDK1, PDPK1, PKCa, PKCb1, PKCe, PKD1, and TBK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Frog.
Related Product 1: Akt1 expression antibody (Cat. No.: AB-NK129-3)
Related Product 2: Akt1 expression antibody (Cat. No.: AB-NK129-4)
Related Product 3: Akt1 expression antibody (Cat. No.: AB-NK129-5)
Related Product 4: Akt1 T308 phosphorylation antibody (Cat. No.: AB-PK515)
Related Product 5: Akt1 Y315 phosphorylation antibody (Cat. No.: AB-PK516)
Related Product 6: Akt1 Y326 phosphorylation antibody (Cat. No.: AB-PK517)
Related Product 7: Akt1 T443 phosphorylation antibody (Cat. No.: AB-PK973)
Related Product 8: Akt1 S473 phosphorylation antibody (Cat. No.: AB-PK869)
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Scientific Background: Akt1 (PKBA) is a protein-serine/threonine kinase of the AGC group and Akt family. It is normally inactive in the cytoplasm. When PI3K is activated by extracellular growth factors, Akt1 is recruited to the cell membrane and phosphorylated by PDK1 (T308) , mTOR (at S473) and other protein kinases to become active. Phosphorylation of T72, S124, S129, S246, T308, Y315,Y326, T450, S473 and Y474 all contribute to increased Akt1 phosphotransferase activity. TRAF6-induced 'K63'-linked Akt1 ubiquitination is critical for phosphorylation and activation. Phosphorylation of T34 inhibits phosphotransferase activity. Akt1 activation leads to the induction of survival, proliferation, angiogenesis and metabolism pathways. Akt1 is also involved in cell-cycle progression via its inhibitory phosphorylation of cyclin-dependent kinases and feedback activatory phosphorylation of mTOR. It phosphorylates over 100 known substrates to inhibit apoptosis and stimulate anabolic processes. PAK1 consists of an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. This kinase is highly expressed in most tested human tissues. Orthologues of Akt are conserved in a wide range of animals, plants and unicellular eukaryotes. Overactive or overexpressed Akt1 is implicated in various cancers (e.g., lymphomas, breast, lung, colorectal, pancreatic and prostate) and is linked to psychiatric conditions like schizophrenia when associated with cannabis use. This description may include information annotated by UniProt and/or Google AI.