Product Name:
PFTAIRE2-pY114
Product Number:
ab-pk957
Target Full Name: Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 7 protein
Target Alias: A2S7; AL2S7; ALS2CR7; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 7 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7; CDK15; PFTAIRE protein kinase 2; PFTK2; Serine/threonine-protein kinase ALS2CR7
Product Type Specific: PFTAIRE2 ( CDK15; ALS2CR7) protein kinase phosphosite-specific antibody
Antibody Code: PK957
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y114
Protein UniProt: Q96Q40
Protein SigNET: PFTAIRE2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PFTAIRE2
Antibody Immunogen Sequence: CGEGS(pY)ATVY
Antibody Immunogen Description: Corresponds to amino acid residues G110 to Y118. The effect of Y114 phosphorylation is unclear, although this is suspected to be inhibitory as it resides in the kinase subdomain I (GXGXXG) region of the kinase. This is the major site of in vivo phosphorylation with ≥ 26 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Mouse.
Related Product 1: PFTAIRE1 expression antibody (Cat. No.: AB-NK287-1)
Related Product 2: PFTAIRE2 expression antibody (Cat. No.: AB-NK004-2)
Related Product 3: PFTAIRE2 expression antibody (Cat. No.: AB-NK004-3)
Scientific Background: PFTAIRE2 (CDK15, PFTK2, ALS2CR7) is a protein-serine/threonine kinase of the CMGC group and CDK family. It controls cell cycle progression. PFTAIRE2 activity is mediated via a cyclin. PFTAIRE2 attenuates TRAIL induced apoptosis in part by phosphorylation of survivin (BIRC5) at Thr-34. It acts as a subunit of the cyclin-dependent protein kinase holoenzyme complex. PFTAIRE2 protein is found in both the cytoplasm and the nucleus. It may be associated with amyotrophic lateral sclerosis 2 (ALS2), which is an autosomal recessive form of juvenile ALS. Elevated expression of PFTAIRE2 is linked to breast cancer progression and poor patient prognosis. It is also involved in colorectal cancer progression via the beta-catenin/MEK-ERK signaling pathway. This description may include information annotated by UniProt and/or Google AI.