Product Name:
PINK1-pT257
Product Number:
ab-pk942
Target Full Name: Serine/threonine-protein kinase PINK1, mitochondrial
Target Alias: Parkinson disease (autosomal recessive) 6; EC 2.7.11.1; PARK6; BRPK; FLJ27236; PTEN induced putative kinase 1; Phosphatase and Tensin Homologue
Product Type Specific: PINK1 protein kinase phosphosite-specific antibody
Antibody Code: PK942
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T257
Protein UniProt: Q9BXM7
Protein SigNET: PINK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PINK1
Antibody Immunogen Sequence: CGAV(pT)YRK
Antibody Immunogen Description: Corresponds to amino acid residues G254 to K260. T257 phosphorylation inhibits cellular apoptosis. This is a minor in vivo phosphorylation site in PINK1 (≥3 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PINK1 is known to be phosphorylated at this site in vitro by PINK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Rat | Mouse.
Related Product 1: PINK1 expression antibody (Cat. No.: AB-NK304-1)
Related Product 2: PINK1 S228 phosphorylation antibody (Cat. No.: AB-PK906)
Related Product 3: PINK1 T313 phosphorylation antibody (Cat. No.: AB-PK907)
Related Product 4: PINK1 S402 phosphorylation antibody (Cat. No.: AB-PK908)
Scientific Background: PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kDa protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kDa and 42 kDa). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports; PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. It regulates axonal transport by phosphorylating mitochondrial Rho GTPase 1 (Miro1) (1). Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction. Downregulation of PINK1 suppressed the TDP- 43-induced degenerative phenotypes in a Drosophila model, and PINK1 also functioned as a genetic modifier of FUS- induced neurodegeneration (2,3). This description may include information annotated by UniProt and/or Google AI.