Product Name:
Nek1-pS1052
Product Number:
ab-pk940
Target Full Name: NIMA (never in mitosis gene a)-related kinase 1
Target Alias: EC 2.7.11.1; KIAA1901; NIMA (never in mitosis gene a)-related kinase 1; NimA-related protein kinase 1; NY-REN-55; NY-REN-55 antigen
Product Type Specific: Nek1 protein kinase phosphosite-specific antibody
Antibody Code: PK940
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S1052
Protein UniProt: Q96PY6
Protein SigNET: NEK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human NEK1
Antibody Immunogen Sequence: RTA(pS)LPD(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R1049 to D1055. The effect of S1052 phosphorylation is unknown. This is the major phosphosite in NEK1 by mass spectrometry (≥112 reports recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog.
Related Product 1: Nek2 S171 phosphorylation antibody (Cat. No.: AB-PK732)
Related Product 2: Nek2 T170+S171 phosphorylation antibody (Cat. No.: AB-PK733)
Related Product 3: Nek6 S206 phosphorylation antibody (Cat. No.: AB-PK734)
Related Product 4: Nek7 T191+S195 phosphorylation antibody (Cat. No.: AB-PK735)
Scientific Background: Nek1 (NIMA-related kinase 1) is a protein serine/threonine protein kinase in the Other Group and NEK family. It also has phosphotransferase activity towards tyrosine residues. It is critical for DNA damage repair, cilia assembly, and cell cycle regulation. NEK1 is highly expressed in germ cells and plays a key role in meiosis. It is involved in DNA damage checkpoint control and for proper DNA damage repair, in part through Nek1 phosphorylation of VDAC1 to limit mitochondrial cell death. It acts in the early response to DNA damage, specifically in repairing double-strand breaks by stabilizing the ATR-ATRIP kinase complex, often operating independently of canonical ATM/ATR mechanisms. It features an N-terminal kinase domain (around residues 4–258) and a large C-terminal extension with coiled-coil regions that mediate protein-protein interactions. It regulates the assembly of primary cilia and maintains centrosome stability. Nek1 has been linked to polycystic kidney disease, short rib-polydactyly syndrome, short-rib thoracic dysplasia 6 with or without Polydactyly, asphyxiating thoracic dystrophy (ATD), sensenbrenner syndrome (CED), and short rib-polydactyly syndrome Type 2. Polycystic Kidney Disease, Type 1 (PKD1) is a rare disease where multiple cysts develop in the kidney, often leading to kidney failure. Missense mutations, including loss of function variants, have been identified in both familial and sporadic ALS (1-5). Besides affecting neuronal morphology and axonal polarity, Nek1 also regulates cellular viability and the permeability of the mitochondrial membrane through phosphorylation of the voltage-dependent anionchannel 1 (VDAC1) (6). Nek1 can also interact with multiple ALS-related gene products, including alsin, VAPB and C21ORF2 (7). This description may include information annotated by UniProt and/or Google AI.