Product Name:
EIF2AK3-pS715
Product Number:
ab-pk927
Target Full Name: Eukaryotic translation initiation factor 2-alpha kinase 3
Target Alias: E2AK1; EC 2.7.11.1; EIF2AK1; HCR; HRI; Heme-controlled repressor; heme-regulated inhibitor; Hemin-sensitive initiation factor-2 alpha kinase
Product Type Specific: EIF2AK3 protein kinase phosphosite-specific antibody
Antibody Code: PK927
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S715
Protein UniProt: Q9NZJ5
Protein SigNET: EIF2AK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human EIF2AK3
Antibody Immunogen Sequence: IIAP(pS)PQR(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues I711 to R718. S715 phosphorylation appears to induce downstream induction of transcription. It appears to be one of the most commonly observed phosphosite in EIF2AK3 from high throughput mass spectrometry studies (≥21 reports). EIF2AK3 is known to be phosphorylated at this site in vitro by AMPKA1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
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Scientific Background: PERK (Protein kinase R-like endoplasmic reticulum kinase; EIF2AK3) is a protein-serine/threonine kinase of the Other group and PEK family. It serves as a critical effector of unfolded protein response-induced G1 growth arrest. Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and induction of its phosphotransferase activity. It mitigates ER stress by phosphorylating eIF2α to reduce protein synthesis and activating survival pathways, playing a crucial role in pancreatic beta-cell function. Under ER stress, the chaperone GRP78 dissociates from PERK, allowing it to oligomerize and autophosphorylate, which activates its cytosolic kinase domain. It features an N-terminal ER luminal stress-sensing domain, a transmembrane segment, and a C-terminal cytosolic kinase domain. It phosphorylates the alpha-subunit of eukaryotic initiation factor 2 (eIF2a) at Ser-51. This action reduces the load of unfolded proteins entering the ER by inhibiting general translation initiation. While global translation is reduced, PERK activation specifically increases the translation of transcription factors like ATF4, which promote cell survival and antioxidant responses. Mutant TDP-43 transgenic flies showed increased eIF2a phosphorylation and an impaired motor function (1). In line with this, knockdown of the fly homologue of PERK improved the motor function in these flies (2). PERK is implicated in pancreatic development, with mutations causing Wolcott-Rallison syndrome. It is also involved in promoting survival in cancer cells and modulating neurodegenerative diseases. This description may include information annotated by UniProt and/or Google AI.