Product Name:
ChaK1-pS1504
Product Number:
ab-pk921
Target Full Name: Transient receptor potential cation channel subfamily M member 7
Target Alias: EC 2.7.11.1; Long transient receptor potential channel 7; LTRPC7; TRPM7
Product Type Specific: CHAK1 (TRPM7) protein kinase phosphosite-specific antibody
Antibody Code: PK921
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S1504
Protein UniProt: Q96QT4
Protein SigNET: ChaK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ChaK1
Antibody Immunogen Sequence: C(βA)RRP(pS)TED
Antibody Immunogen Description: Corresponds to amino acid residues R1501 to D1507. The effect of S1504 phosphorylation is unclear. This is the major site of in vivo phosphorylation with ≥ 333 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken.
Scientific Background: TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants (1, 2). K1648R and G1799D mutations lead to loss of activity for this atypical protein-serine/threonine kinase and are associated with amyotrophic lateral sclerosis-parkinsonism/dementia complex 1 (ALS-PDC1), which is characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. This description may include information annotated by UniProt and/or Google AI.