Product Name:
TrkB-pY706+pY707
Product Number:
ab-pk917
Target Full Name: BNDF/NT3/4/5 receptor- tyrosine kinase
Target Alias: GP145-TrkB; GP145-TrkB,GP95-TrkB; NTrk2; CCDS6671.1; ENSG00000148053
Product Type Specific: TrkB protein kinase phosphosite-specific antibody
Antibody Code: PK917
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y706+Y707
Protein UniProt: Q16620
Protein SigNET: TrkB
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human TrkB
Antibody Immunogen Sequence: STD(pY)(pY)RVGGC
Antibody Immunogen Description: Corresponds to amino acid residues S703 to G711. Y706 and Y707 phosphorylation stimulates phosphotransferase activity and induces interaction with PTP1B. These phosphosites are located in the kinase activation loop between catalytic subdomains VII and VIII. This are major sites of phosphorylation for TrkB based on ≥49 and ≥27 high throughput mass spectrometry reports, respectively, recorded in PhosphoSitePlus. TrkB is known to be phosphorylated at this site in vitro by Y706 at Y707 by Src, and TrkB (NTRK2).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish.
Related Product 1: TrkSubtide kinase substrate peptide (Cat. No.: PE-01BJQ95)
Related Product 2: TrkA Y680+Y681 phosphorylation antibody (Cat. No.: AB-PK837)
Related Product 3: TrkB Y516 phosphorylation antibody (Cat. No.: AB-PK838)
Related Product 4: TrkB Y702 phosphorylation antibody (Cat. No.: AB-PK839)
Related Product 5: TrkC Y709+Y710 phosphorylation antibody (Cat. No.: AB-PK840)
Scientific Background: TrkB is one of several protein kinases that can phosphorylate KinSub5DDDYV. TrkB is a receptor protein-tyrosine kinase that is a member of the TK group of protein kinases in the Trk family. This kinase shows high variability in human tissue distribution with the highest levels detected in brain, ovary, pancreas, pituitary, spinal cord, thyroid and trachea. TrkB is activated by binding BDNF, NT-3, NT-4 or NT-5, which induce dimerization and autophosphorylation. Phosphorylation at Y516 increases phosphotransferase activity and induces interaction with PLCg1. TrkB activation stimulates signalling throught the MAPK pathway. Signalling through TrkB leads to cell differentiation. Mutations in the TrkB gene have been associated with obesity and mood disorders. TrkB has also been linked with the development of colon and lung cancers, neuroblastomas and pilocytic astrocytomas, which are benign tumours of the brain or spinal cord that grow slowly. This description may include information annotated by UniProt and/or Google AI.