Product Name:
STLK3-pT231
Product Number:
ab-pk916
Target Full Name: STE20-SPS1-related proline-alanine rich protein kinase
Target Alias: DCHT; EC 2.7.11.1; Serine threonine kinase 39; Serine/threonine-protein kinase 39; SPAK; Ste-20 related kinase; STE20/SPS1-related proline-alanine rich protein kinase
Product Type Specific: STLK3 (STK39) protein kinase phosphosite-specific antibody
Antibody Code: PK916
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T231
Protein UniProt: Q9UEW8
Protein SigNET: STLK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human STLK3
Antibody Immunogen Sequence: VRK(pT)FVGC
Antibody Immunogen Description: Corresponds to amino acid residues V228 to G234. T231 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is a major site of in vivo phosphorylation based on ≥55 mass spectrometry reports recorded in PhosphoSitePlus. STLK3 is known to be phosphorylated at this site in vitro by STLK3, and Wnk1 (PRKWNK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse | Platypus | Chicken | Fruit fly | Honey bee | Sea urchin.
Related Product 1: STLK6 S304+S306 phosphorylation antibody (Cat. No.: AB-PN911)
Scientific Background: STLK3 (STK39; SPAK) is a protein-serine/threonine kinase in the STE Group, STE20 family and FRAY subfamily. It is a component of the WNK-SPAK/OSR1 kinase cascade, which is implicated in ion transport, and responses to hypertonic stress and blood pressure. It mediates the activation of the p38 MAP kinase pathway, and primarily functions as a mediator that phosphorylates cation-chloride-coupled cotransporters, affecting cell volume regulation and blood pressure control. It is phosphorylated by WNK1, WNK2, WNK3 or WNK4, and in turn phosphorylates ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3. It phosphorylates and activates Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 to promote ion influx; phosphorylates and inactivates K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 to block ion efflux. STLK3 is predominantly expressed in the brain and pancreas, with lower levels in the heart, lung, kidney, skeletal muscle, liver, placenta, and testis. This description may include information annotated by UniProt and/or Google AI.