Product Name:
PKR-pT451
Product Number:
ab-pk910
Target Full Name: Double-stranded RNA-dependent protein-serine kinase; Interferon-induced, double-stranded RNA-activated protein kinase
Target Alias: ADRB2; E2AK2; EIF2aK; EIF2AK1; EIF2AK2; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-inducible RNA-dependent protein kinase; P1,eIF-2A protein kinase; TIK; MGC126524; ENSG00000055332
Product Type Specific: PKR1 protein kinase phosphosite-specific antibody
Antibody Code: PK910
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T451
Protein UniProt: P19525
Protein SigNET: PKR
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PKR
Antibody Immunogen Sequence: CRSKG(pT)LRY
Antibody Immunogen Description: Corresponds to amino acid residues R447 to Y454. T451 phosphorylation stimulates phosphotransferase activity. This is a minor in vivo phosphorylation sites in PKR1 (≥1 report from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PKR1 is known to be phosphorylated at this site in vitro by ERK2 (MAPK1), p38a, PKR, and RSK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Mouse.
Related Product 1: PKR1 T446 phosphorylation antibody (Cat. No.: AB-PK132)
Related Product 2: PKR1 T446 phosphorylation antibody (Cat. No.: AB-PK777)
Scientific Background: PKR (PRKR, EIF2AK1) is a protein-serine/threonine kinase of the Other group and PEK family. It has an antiviral activity on various ranges of DNA and RNA viruses such as hepatitis C virus, hepatitis B virus, measles virus, and herpes simplex virus 1. It is activated by autophosphorylation after binding to dsRNA (generated during virus infection) in the presence of ATP. Besides double stranded RNA (dsRNA), heparin is a potent activator of the kinase. Binding to dsRNA is required for dimerization leading to autophosphorylation in the activation loop and stimulation of function. Activity is markedly stimulated by manganese ions. Autophosphorylation at S83, T88, T89, T90, Y101, Y162, S242, T255, T258, Y293 T446, T451, and S456 increase the phosphotransferase activity of EIF2AK2. Once activated, PKR can phosphorylate translation initiation factor EIF2S1, which in turn will inhibit protein synthesis. It can be inhibited by vaccinia virus protein E3, possibly via dsRNA sequestering. It has a role in p53-mediated responses to genotoxic stress. Since the protein has a crucial role for inflammation activation, it can be a potential therapeutic target to treat inflammation. This description may include information annotated by UniProt and/or Google AI.