Product Name:
LRRK2-pS935
Product Number:
ab-pk905
Target Full Name: Leucine-rich repeat protein-serine/threonine kinase 2
Target Alias: Leucine-rich repeat kinase 2; RIP7; PARK8; Dardarin; ROCO 2; RIPK7; augmented in rheumatoid arthritis 17
Product Type Specific: LRRK2 protein kinase phosphosite-specific antibody
Antibody Code: PK905
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S935
Protein UniProt: Q5S007
Protein SigNET: LRRK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human LRRK2
Antibody Immunogen Sequence: HSN(pS)LGP(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues H932 to P938. S935 phosphorylation induces interaction with 14-3-3-beta, 14-3-3-epsilon, 14-3-3-eta, 14-3-3-theta, and 14-3-3-zeta, and regulates intracellular location. This is a major in vivo phosphorylation site in LRRK2 (≥19 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). LRRK2 is known to be phosphorylated at this site in vitro by PKACa (PRKACA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse.
Related Product 1: LRRKtide KinSub kinase substrate peptide (Cat. No.: PE-01ADD95)
Related Product 2: LRRKSubtide kinase substrate peptide (Cat. No.: PE-01BHX95)
Related Product 3: LRRK2/Dardarin expression antibody (Cat. No.: AB-NK303-1)
Related Product 4: LRRK2/Dardarin expression antibody (Cat. No.: AB-NK303-2)
Related Product 5: LRRK2 S955 phosphorylation antibody (Cat. No.: AB-PK935)
Related Product 6: LRRK2 S973 phosphorylation antibody (Cat. No.: AB-PK936)
Related Product 7: LRRK2 T1410 phosphorylation antibody (Cat. No.: AB-PK937)
Related Product 8: LRRK2 T1503 phosphorylation antibody (Cat. No.: AB-PK938)
Related Product 9: LRRK2 T2031+S2032 phosphorylation antibody (Cat. No.: AB-PK939)
Scientific Background: LRRK2 (Leucine-rich repeat kinase 2; Dardarin) is a 268 kDa protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named ROC, and a MAPKKK kinase domain that is closely related to the RIP kinase domain. It acts as a signaling molecule and scaffold protein, phosphorylating a subgroup of Rab GTPases (e.g., Rab8A, Rab10) to regulate intracellular trafficking and lysosomal functions. LRRK2 positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signalling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. It is found in the cytoplasm and associated with membranes of organelles such as lysosomes, endosomes, and the Golgi apparatus. LRRK2 is expressed in brain as well as in other tissues such as lung, liver and heart. It is also highly expressed in immune cells, particularly neutrophils. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with Parkinsonism. Mutations (e.g., G2019S, R1441G) typically cluster in the catalytic ROC-COR-Kinase core, increasing kinase activity. Increased kinase activity disrupts cellular trafficking and autophagic processes, leading to neurodegeneration. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features. LRRK2 is also linked to Crohn's disease and Hansen’s disease. This description may include information annotated by UniProt and/or Google AI.