Product Name:
JNK2-pT183+pY185
Product Number:
ab-pk902
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinase (SAPK)) 2
Target Alias: c-Jun N-terminal kinase 2; JNK2alpha; JNK2-alpha-2; JNK2A; JNK2B; JNK2beta; JNK-55; Jun kinase; Kinase JNK2; MAPK9; MK09; p54a; PRKM9; Stress-activated protein kinase JNK2; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; CCDS4453.1; ENSG00000050748
Product Type Specific: JNK2 (MAPK9) protein kinase phosphosite-specific antibody
Antibody Code: PK902
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T183+Y185
Protein UniProt: P45984
Protein SigNET: JNK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human JNK2
Antibody Immunogen Sequence: FMM(pT)P(pY)VV(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues F180 to V187. Phosphorylation of T183+Y185 increase the phosphotransferase activity of JNK2. These are the major in vivo phosphorylation sites in JNK2 (≥455 and ≥1477 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). JNK2 is known to be phosphorylated in vitro at T183 by MKK7, and at Y185 by MKK4, and RET.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Frog.
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Scientific Background: JNK2 (MAPK9) is a protein-serine/threonine kinase of the CMGC group and MAPK family. JNK2 is expressed as several isoforms (46 kDa and 54 kDa) through alternative splicing, mainly differing in their C-terminus. It is involved in the control of cell differentiation, transformation, migration, proliferation, and programmed cell death in response to stress and cytokines. JNK2 is known to play a critical role in T-cell differentiation and apoptosis, by preventing ubiquitination of p53 in non-stress cells. Phosphorylation of Thr-183 and Tyr-185 by either of two dual specificity kinases, MAP2K4 and MAP2K7, increases phosphotransferase activity. Phosphorylation of Ser-129 also increases phosphotransferase activity. It is inhibited by dual specificity phosphatases, such as DUSP1, which target the Thr-183 and Tyr-185 activatory phosphorylation sites. JNK2 often functions as a positive regulator of c-Jun, though it can also act as a negative regulator of cell proliferation, unlike JNK1. JNK2 has been linked to several pathologies, including cancer (e.g., lung carcinoma transformation) and inflammation. Studies also indicate its role in promoting cell survival during endoplasmic reticulum (ER) stress. JNK2 has been shown, in a cancer cell line to have a role in breast cancer cell motility. This description may include information annotated by UniProt and/or Google AI.