Product Name:
DAPK1-pS308
Product Number:
ab-pk900
Target Full Name: Mitogen-activated protein kinase kinase kinase 12
Target Alias: Dual leucine zipper bearing kinase; Dual leucine zipper kinase DLK; EC 2.7.11.25; Kinase DLK; Leucine-zipper protein kinase; M3K12; MAP3K12; MAPK-upstream kinase; MEKK12; MUK; ZPK; ZPKP1
Product Type Specific: DAPK protein kinase phosphosite-specific antibody
Antibody Code: PK900
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S308
Protein UniProt: P53355
Protein SigNET: DAPK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human DAPK1
Antibody Immunogen Sequence: WKQ(pS)VRL(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues W305 to L311. S308 phosphorylation inhibits phosphotransferase activity. This is one of the minor in vivo phosphorylation sites in DLK (DAPK1) (≥4 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). DLK is known to be phosphorylated at this site in vitro by DAPK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse | Frog | Zebra fish.
Related Product 1: DAPK S269 phosphorylation antibody (Cat. No.: AB-PK590)
Scientific Background: DAPK1 (Death-associated protein kinase 1) is a 160 kDa calcium/calmodulin-dependent serine/threonine kinase that belongs to the CAMK Ser/Thr protein kinase family, DAP kinase subfamily. It features an N-terminal kinase domain, 8 ankyrin repeats, and a C-terminal death domain. DAPK1 is activated by Ca2+/calmodulin binding, and it is regulated by a "locking" mechanism involving dephosphorylation at Ser-308. It functions in multiple cellular signalling pathways that trigger cell survival, apoptosis, and autophagy. It regulates both type I apoptotic (caspase-dependent) and type II autophagic (caspase-independent) cell death signalling, depending on the cellular setting. It phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylation of TPM1 by DAPK1, enhances stress fiber formation in endothelial cells. STX1A phosphoryation by DAPK1 significantly decreases its binding to STXBP1. It phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. BECN1 phosphorylation by DAPK1 reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. It phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Other substrates for DAPK1 include RPS6, MYL9 and DAPK3. It acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. It is required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which induces RPL13A involvement in transcript-selective translation inhibition. Isoform 2 cannot induce apoptosis but can induce membrane blebbing. Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas. Upregulation of DAPK1 is linked to Alzheimer's disease, promoting neuronal apoptosis and tau hyperphosphorylation. This description may include information annotated by UniProt and/or Google AI.