Product Name:
CDK5-pS159
Product Number:
ab-pk899
Target Full Name: Cyclin-dependent protein-serine kinase 5
Target Alias: Cell division protein kinase 5; CRK6; PSSALRE; TAU protein kinase II; TPKII; ENSG00000164885
Product Type Specific: CDK5 protein kinase phosphosite-specific antibody
Antibody Code: PK899
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S159
Protein UniProt: Q00535
Protein SigNET: CDK5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CDK5
Antibody Immunogen Sequence: RSY(pS)AEV(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R156 to V162. S159 phosphorylation is stimulatory for phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the minor in vivo phosphorylation sites in CDK5. CDK5 is known to be phosphorylated at this site in vitro by CK1a.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Sea urchin.
Related Product 1: CDK5 (12-18) pY15 antibody blocking peptide (Cat. No.: PE-04AJO95)
Related Product 2: CDK5Selectide kinase substrate peptide (Cat. No.: PE-01BGQ95)
Related Product 3: CDK5 expression antibody (Cat. No.: AB-NK028-2)
Related Product 4: CDK5 Y15 phosphorylation antibody (Cat. No.: AB-PK570)
Scientific Background: CDK5 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is required for cell cycle arrest in neuronal cells. It regulates neurite outgrowth, neuronal differentiation, and synaptic vesicle recycling. CDK5 is involved in cell motility, survival, apoptosis, and angiogenesis in non-neuronal tissues. It is activated by binding p39 and it truncated p35 protein. Phosphorylation at Ser-159 increases phosphotransferase activity. Phosphorylation at Thr-14 and Tyr-15 inhibits phosphotransferase activity. CDK5 has been observed to be proline-directed and to phosphorylate SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. CDK5 has been implicated in Aneurysmal bone cyst, which is a bone cancer that will affect bone, endothelial tissue, and bone marrow. Many neurological disorders appear to include targets for CDK5, such as Tau in Alzheimer's disease. This description may include information annotated by UniProt and/or Google AI.