Product Name:
PIK3CA-pY317
Product Number:
ab-pk894
Target Full Name: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Target Alias: EC 2.7.1.153; Phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3K; PI3K p110-alpha; PI3-kinase p110 alpha; PK3CA; PtdIns-3-kinase p110
Product Type Specific: PIK3CA protein kinase phosphosite-specific antibody
Antibody Code: PK894
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y317
Protein UniProt: P42336
Protein SigNET: PIK3CA
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PIK3CA
Antibody Immunogen Sequence: ATP(pY)MNGC
Antibody Immunogen Description: Corresponds to amino acid residues A314 to N319. The effect of Y317 phosphorylation is unclear. This is the major in vivo phosphorylation site in PIK3CA (≥23 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in fish.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Mouse | Platypus | Chicken | Zebra fish.
Related Product 1: PIK3R1 Y580 phosphorylation antibody (Cat. No.: AB-PN527)
Related Product 2: PIK3R2 Y464 phosphorylation antibody (Cat. No.: AB-PN528)
Scientific Background: PIK3CA (Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3K) is a lipid kinase that belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. It participates in cellular signaling in response to various growth factors. It is involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. It is involved in signaling via insulin-receptor substrate (IRS) proteins. PIK3CA is essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. It is required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. It regulates invadopodia formation through the PDPK1-AKT1 pathway. It participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. It participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. PIK3CA also has serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. It plays a role in the positive regulation of phagocytosis and pinocytosis. Mutations in PIK3CA are frequently associated with cancers (breast, colon, etc.) and overgrowth syndromes. Mutations, such as H1047R and E545K, cause overactivation of the enzyme, driving tumour growth in numerous cancers. Activating mutations can cause PIK3CA-Related Overgrowth Spectrum (PROS), a group of rare conditions causing overgrowth of tissue, bones, or blood vessels. This description may include information annotated by UniProt and/or Google AI.