Product Name:
VACAMKL-pY245
Product Number:
ab-pk892
Target Full Name: CaM kinase-like vesicle-associated protein
Target Alias: CaM kinase-like vesicle-associated; CAMKV; MGC8407
Product Type Specific: VACAMKL protein kinase phosphosite-specific antibody
Antibody Code: PK892
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y245
Protein UniProt: Q8NCB2
Protein SigNET: VACAMKL
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human VACAMKL
Antibody Immunogen Sequence: CAGD(pY)EFD
Antibody Immunogen Description: Corresponds to amino acid residues A242 to D248. The effect of Y245 phosphorylation is unknown. Y245 phosphorylation has been observed in only a small number of mass spectrometry studies (~6 reports recorded in PhosphoSitePlus). The effect of its phosphorylation is unknown. However, it has homology with the Y25 phosphosite in CaMK1a. VACAMKL has been suggested to not have detectable phosphotransferase activity.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse | Frog | Nematode worm.
Scientific Background: VACAMKL (CaM kinase-like vesicle-associated protein; CAMKV) is a protein-serine/threonine kinase that belongs to the CAMK Ser/Thr protein kinase family. Despite its structural classification, it may function as a pseudokinase, since it lacks critical residues thought to be important for phosphotransferase activity. However, since it binds calmodulin and it may be under calcium control. It is expressed primarily in the nervous system. it is necessary for the activity-dependent maintenance of dendritic spines, and may be involved in neurotransmission with potential roles in neurodevelopment. It is predominantly found in the brain (forebrain), localized to the cytoplasm, plasma membrane, and on cytoplasmic vesicles, particularly in neurons and neurites. This description may include information annotated by UniProt and/or Google AI.