Product Name:
MYT1-pS143
Product Number:
ab-pk887
Target Full Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
Target Alias: EC 2.7.11.1; Myt1 kinase; PMYT1
Product Type Specific: MYT1 protein kinase phosphosite-specific antibody
Antibody Code: PK887
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S143
Protein UniProt: Q99640
Protein SigNET: MYT1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human MYT1
Antibody Immunogen Sequence: RSM(pS)PFR(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R140 to R146. The effect of S143 phosphorylation is unknown. S143 is the most common phosphosite in MYT1 by mass spectrometry (≥23 reports recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse.
Scientific Background: MYT1 (PKMYT1) is a protein-serine/threonine kinase in the Other Group and WEE family. It serves as a negative regulator of entry into mitosis and meoisis (G2 to M transition) by phosphorylation of the CDK1 kinase at Thr-14 specifically when CDK1 is complexed to cyclins. To a lesser extent, it may directly or indirect also catalyze the phosphorylation of Tyr-15. Phosphorylation of CDK1 prevents premature mitosis, and is essential for Golgi/ER assembly during telophase. It is mainly found associated with the endoplasmic reticulum and Golgi complex, rather than in the nucleus. MYT1 is linked to Dysembryoplastic Neuroepithelial tumours (DNT, DNET), which are rare brain tumours that often affect the temporal lobe or the caudate nucleus. DNT is often benign, but can lead to epilepsy. This description may include information annotated by UniProt and/or Google AI.