Product Name:
ERK2-pY263+S266
Product Number:
ab-pk880
Target Full Name: Extracellular regulated protein-serine kinase 2 (p42 MAP kinase)
Target Alias: ERK; ERK-2; ERT1; Extracellular signal regulated kinase 2; Kinase ERK2; MAP kinase 2; MAP kinase isoform p42; MAPK; MAPK 2; PRKM2; p41mapk; CCDS13795.1; ENSG00000100030
Product Type Specific: ERK2 protein kinase phosphosite-specific antibody
Antibody Code: PK880
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y263+S266
Protein UniProt: P28482
Protein SigNET: ERK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ERK2
Antibody Immunogen Sequence: ARN(pY)LL(pS)LPH(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues A260 to H269. The effects of Y263 and S266 phosphorylation are unknown. Phosphorylation of Y263 has only been observed once in a mass spectrometry studies with human ERK2, and phosphorylation of S266 has not been reported.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Honey bee | Nematode worm | Sea urchin.
Related Product 1: ERK2 expression antibody (Cat. No.: AB-NK056-2)
Related Product 2: ERK2 expression antibody (Cat. No.: AB-NK056-3)
Related Product 3: ERK2 expression antibody (Cat. No.: AB-NK056-4)
Related Product 4: ERK2 T185+Y187 phosphorylation antibody (Cat. No.: AB-PK622)
Related Product 5: ERK2 Y263+S266 phosphorylation antibody (Cat. No.: AB-PK880)
Related Product 6: ERK1 T202+Y204 phosphorylation antibody (Cat. No.: AB-PK621)
Related Product 7: ERK1 Y204 phosphorylation antibody (Cat. No.: AB-PK864)
Related Product 8: ERK1 T202 phosphorylation antibody (Cat. No.: AB-PK967)
Related Product 9: ERKSelectideA - ERK1 (MAPK3) protein kinase substrate peptide
Related Product 10: ERKSelectideB - ERK1 (MAPK3) protein kinase substrate peptide
Scientific Background: ERK2 (MAPK1) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is involved in the MAP Kinase signalling pathway. The MAP kinase pathway can mediate cell adhesion, growth, survival, differentiation, meiosis, mitosis, and other post-mitotic functions. Phosphorylation of T185 and Y187 by MEK1 an MEK2 increases its phosphotransferase activity. It phosphorylates many diffrent transcription factors, such as ELK1. It acts as transcriptional repressor by binding directly to DNA. It is essential for cyclin D1 induction. MAPK1 phosphorylates BCL2, which contributes to cell survival, the suppression of the apoptotic effect of BAD and up-regulation of the antiapoptotic protein MCL-1. It regulates accumulation of p53 during DNA damage response. ERK2 appears to be a tumour requiring protein (TRP). The active form of the protein kinase normally acts to promote tumour cell proliferation. ERK2 has been linked with the Ewing's Family of tumours, which are rare cancers that are similar to medullablastoma and cerebral primitive neuroectodermal tumours. It is constitutively active in many human tumours, primarily due to altered Ras, Raf, EGFR or other upstream oncoproteins. This description may include information annotated by UniProt and/or Google AI.