Product Name:
MuSK-pY756
Product Number:
ab-pk872
Target Full Name: Muscle, skeletal receptor tyrosine protein kinase
Target Alias: Muscle specific tyrosine kinase receptor
Product Type Specific: MuSK protein kinase phosphosite-specific antibody
Antibody Code: PK872
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y756
Protein UniProt: O15146
Protein SigNET: MuSK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MuSK sequence peptide Cat. No.: PE-04ABM80
Antibody Immunogen Sequence: SADY(pY)KAN(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues S752 to N759; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. Y756 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This phosphorylation site in MUSK has ony been reported in ≥2 high throughput mass spectrometry study. MuSK is known to be phosphorylated at this site in vitro by MUSK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Mouse | Platypus | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100 kDa.
Related Product 1: MuSK (752-759) pY756 antibody blocking peptide (Cat. No.: PE-04ABM80)
Related Product 2: MuSK Y554 phosphorylation antibody (Cat. No.: AB-PK953)
Scientific Background: MuSK is a 120 kDa single-pass transmembrane receptor protein-tyrosine kinase of the TK group of protein kinases in the Musk family. As a type I glycoprotein, it consists of an extracellular region (three immunoglobulin-like domains [Ig1-3] and a cysteine-rich domain [CRD]), a single-pass transmembrane domain, and a cytoplasmic tyrosine kinase domain. Unlike typical kinases, MuSK activation is complex, requiring ligand LRP4, neural agrin, and the intracellular adaptor protein Dok-7. Musk interacts with Dok7, which may regulates its phosphotransferase activity. Dok7 is essential for MuSK activation in cultured myotubes. It triggers postsynaptic acetylcholine receptor (AChR) clustering on the postsynaptic membrane of the neuromuscular junction. MuSK is moderate to highly expressed in most tested human tissues except in the bladder, brain, pericardium, pituitary, spinal cord, trachea and vagina where it is notably low. MuSK is necessary for neuromuscular junction formation. Musk gene expression is highly regulated during neuromuscular junction and it is involved in intercellular communication present on the surface of cells that are activated by specific protein ligands. MuSK members play a key role in growth and differentiation of those cell types. MuSK also plays a critical role in the development of normal blood vessels. Mutations in the MUSK gene can lead to Congenital Myasthenic Syndromes (CMS). Musk has been linked with the development of lung neuroendocrine carcinomas. Autoantibodies against MuSK cause a subset of myasthenia gravis, causing severe muscle weakness. This description may include information annotated by UniProt and/or Google AI.