Product Name:
MST1-pT183
Product Number:
ab-pk871
Target Full Name: Mammalian STE20-like protein-serine kinase 1 (KRS2); Serine-threonine-protein kinase 4
Target Alias: DKFZp686A2068; Kinase MST1; Kinase responsive to stress 2; KRS2; Krs-2; Mammalian sterile 20-like 1; MST-1; Serine,threonine protein kinase 4; STK4; YSK3; DKFZp686A2068; CCDS13341.1; ENSG00000101109
Product Type Specific: MST1 protein kinase phosphosite-specific antibody
Antibody Code: PK871
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T183
Protein UniProt: Q13043
Protein SigNET: MST1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MST1 (STK4, Krs2) sequence peptide Cat. No.: PE-04AQW95
Antibody Immunogen Sequence: AKRN(pT)VIGT(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues A179 to T187. T183 stimulates phosphotransferase activity. Phosphorylation induces protein ubquitination and degradation, and regulates molecular association. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the minor in vivo phosphorylation sites in MST1 (≥6 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). MST1 is known to be phosphorylated at this site in vitro by MST1 (STK4, KRS2).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Nematode worm | Sea urchin.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 53-57 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer, although strong background on gels >75 kDa was evident.
Related Product 1: MST1 (179-187) pT183 antibody blocking peptide (Cat. No.: PE-04AQW95)
Related Product 2: IRS1 (979-989) KinSub - Insulin receptor substrate 1 (K979-G989, mouse) peptide; Insulin receptor substrate
Related Product 3: MstSubtide kinase substrate peptide (Cat. No.: PE-01BII95)
Related Product 4: MST3 T184 phosphorylation antibody (Cat. No.: AB-PK728)
Related Product 5: MST3 T187 phosphorylation antibody (Cat. No.: AB-PK727)
Scientific Background: MST1 (STK4; KRS2) is a protein-serine/threonine kinase of the STE group and STE20 family, and is a core component of the Hippo signalling pathway. It features an N-terminal kinase domain, a central regulatory domain, and a C-terminal SARAH domain for binding other Hippo components. It can induce apoptosis following stress activation. It is often found in the cytoplasm, but MST1 relocates to the nucleus upon activation. MST1 activity can occur in the nucleus and can be via condensation of chromatin and fragmentation of intranucleosomal DNA. It forms homodimers and interacts with proteins like RASSF1A and SAV1 for activation, while Akt can phosphorylate and inhibit it. It is activated by caspase-cleavage. Full activation requires homodimerization and autophosphorylation of Thr-183; MST1 phosphotransferase activity can be lost with a T183A mutation. Proteolytic cleavage of MST1 during apoptosis can be reduced with the D326N and D349N mutations. Homodimerization and autophosphorylation are lost with a L444P mutation. It leads to the inactivation of the YAP1 oncoprotein by activating downstream kinases such as LATS1 and LATS2, which phosphorylate YAP1 to prevent it from translocating to the nucleus and transcribing cell proliferation and migration genes. MST1 can act as a tumour suppressor protein by promoting apoptosis by preventing the deacetylation of p53/TP53, resulting in increased p53-dependent transcription and apoptosis. It is also able to inhibit the PKB/Akt1 pathway. Upon cleavage by caspases (e.g., in response to stress), the activated form of MST1 enters the nucleus to trigger chromatin condensation and DNA fragmentation. Deficiency has been linked to severe T-cell lymphopenia and immunodeficiency. MST1 is linked to pancreatic cancer, which is a rare cancer that does not have characteristic symptoms but is related to the integrated breast cancer and pancreatic cancer pathways. This description may include information annotated by UniProt and/or Google AI.