Product Name:
MSK2-pT194 + pS196
Product Number:
ab-pk868
Target Full Name: Ribosomal protein S6 kinase alpha-4
Target Alias: Kinase MSK2; KS6A4; Ribosomal protein kinase B; Ribosomal protein S6 kinase, 90kDa, polypeptide 4; RPS6KA4; RSK-B; Similar to ribosomal protein S6 kinase, 90kD, polypeptide 4; Similar to ribosomal protein S6 kinase, polypeptide 4; CCDS8073.1; ENSG00000162302
Product Type Specific: MSK2 protein kinase phosphosite-specific antibody
Antibody Code: PK868
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T194+S196
Protein UniProt: O75676
Protein SigNET: MSK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MSK2 (RPS6KA4) sequence peptide Cat. No.: PE-04AQX85
Antibody Immunogen Sequence: KER(pT)F(pS)FCGC
Antibody Immunogen Description: Corresponds to amino acid residues K191 to G199; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T194 and S196 phosphorylation stimulates phosphotransferase activity. These are two of the minor in vivo phosphorylation sites in MSK2 (≥1 and ≥2 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). MSK2 is known to be phosphorylated in vitro at S196 by MSK2 (RPS6KA4), and p38a MAPK (MAPK14).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 83-90 kDa.
Related Product 1: MSK2 (191-199) pT194+pS196 antibody blocking peptide (Cat. No.: PE-04AQX85)
Related Product 2: MskSubtide - MSK1 (RPS6KA5) protein kinase substrate peptide
Related Product 3: MSK1 S212 phosphorylation antibody (Cat. No.: AB-PK723)
Related Product 4: MSK2 T687 phosphorylation antibody (Cat. No.: AB-PK725)
Scientific Background: MSK2 (RPS6KA4) is a 114 kDa protein-serine/threonine kinase of the AGC group and RSK family. It is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. It phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. It plays an essential role in the control of RELA transcriptional activity in response to TNF. It phosphorylates Ser-10 as well as Ser-28 of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. It mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. It appears to induce activation of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. F709A substitution is associated with strongly elevated basal activity. MSK2 acts as a downstream effector of the MAPK/ERK and p38 signaling pathways, and is activated by phosphorylation at Ser-196, Ser-343, Ser-347, Thr-360, Thr-568 and Thr-687. Aberrant activation of members of the MSK family have found to be linked with many human diseases, such as breast and prostate cancers. MSK2 acts as an inhibitor of the p53 tumour suppressor protein, thereby inhibiting p53-mediated apoptosis. It has been linked to potential roles in neurodegeneration and inflammation as well. This description may include information annotated by UniProt and/or Google AI.