Product Name:
YSK1-pT174
Product Number:
ab-pk859
Target Full Name: Serine/threonine-protein kinase 25
Target Alias: DKFZp686J1430; SOK1; ST25; Ste20,oxidant stress response kinase-1; Ste20-like kinase; Sterile 20,oxidant stress-response kinase 1; STK25
Product Type Specific: YSK1 protein kinase phosphosite-specific antibody
Antibody Code: PK859
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T174
Protein UniProt: O00506
Protein SigNET: YSK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human YSK1 (STK25, SOK1) sequence peptide Cat. No.: PE-04ALL99
Antibody Immunogen Sequence: KRN(pT)FVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K171 to G177; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T174 phosphorylation is stimulatory for phosphotransferase activity. This is the major site of in vivo phosphorylation of YSK1 based on ≥158 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells with insulin-treatment.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Nematode worm.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-55 kDa.
Antibody Specificity: High to very high
Antibody Cross Reactivity: Strongly cross-reactive ~75-80 kDa protein detected in sea star oocytes and MCF7 cells as well as a ~21 kDa cross-reactive protein in MCF7 cells.
Related Product 1: YSK1 (171-177) pT174 antibody blocking peptide (Cat. No.: PE-04ALL99)
Related Product 2: YSK4 expression antibody (Cat. No.: AB-NK256-1)
Related Product 3: YSK4 expression antibody (Cat. No.: AB-NK256-2)
Related Product 4: YSK4 expression antibody (Cat. No.: AB-NK256-3)
Scientific Background: YSK1 (Yeast Sps1/Ste20-related Kinase 1; STK25) is a protein-serine/threonine kinase that is oxidant stress activated and hypothesized to play a role in the cellular response to environmental stress. YSK1 is activated by the Golgi matrix protein GM130. It controls Golgi apparatus reorganization, which is essential for protein transport events, cell adhesion, and polarity complexes involved in cellular migration.It is part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes, which regulate multiple pathways including Hippo signaling and cytoskeletal remodeling. YSK1 phosphorylates and activates ATG4B at Ser-383, which increases autophagic flux. It also plays a role in regulating apoptotic processes (cell death). Its C-terminal non-catalytic region inhibits YSK1's phosphotransferase activity. Unlike many other Ste20-related kinases, YSK1 lacks the Cdc42/Rac1-binding domain. It is ubiquitously expressed in various tissues but shows high expression in the brain and testis. Genetic inhibition of YSK1 has been shown to improve metabolic health in models of type 2 diabetes. YSK1 is often upregulated in cancers (e.g., prostate cancer) and may contribute to tumor progression. This description may include information annotated by UniProt and/or Google AI.