Product Name:
YSK1-pT174
Product Number:
ab-pk859
Target Full Name: Serine/threonine-protein kinase 25
Target Alias: DKFZp686J1430; SOK1; ST25; Ste20,oxidant stress response kinase-1; Ste20-like kinase; Sterile 20,oxidant stress-response kinase 1; STK25
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK859
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T174
Protein UniProt: O00506
Protein SigNET: O00506
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human YSK1 (STK25, SOK1) sequence peptide Cat. No.: PE-04ALL99
Antibody Immunogen Sequence: KRN(pT)FVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K171 to G177; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues K171 to G177; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells with insulin-treatment.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-55 kDa.
Antibody Specificity: High to very high
Antibody Cross Reactivity: Strongly cross-reactive ~75-80 kDa protein detected in sea star oocytes and MCF7 cells as well as a ~21 kDa cross-reactive protein in MCF7 cells.
Related Product 1: YSK1-pT174 blocking peptide
Scientific Background: YSK1 (STK25) is a protein-serine/threonine kinase that is oxidant stress activated and hypothesized to play a role in the cellular response to environmental stress.YSK1 is specifically targeted to the Golgi apparatus where it is thought to function in the regulation of protein transport events, cell adhesion, and polarity complexes involved in cellular migration. Its C-terminal non-catalytic region inhibitsYSK1's phosphotransferase activity.