Product Name:
Yes-pY222+pY223
Product Number:
ab-pk858
Target Full Name: Yamaguchi sarcoma proto-oncogene-encoded tyrosine kinase
Target Alias: c-Yes; HsT441; p61-Yes; CCDS11824.1; ENSG00000176105
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK858
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y222+Y223
Protein UniProt: P07947
Protein SigNET: P07947
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Yes sequence peptide Cat. No.: PE-04AHY99
Antibody Immunogen Sequence: NGG(pY)(pY)ITT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N219 to T226; In the SH2 domain. One of the two major in vivo phosphorylation sites in Yes.
Production Method: Corresponds to amino acid residues N219 to T226; In the SH2 domain. One of the two major in vivo phosphorylation sites in Yes.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-65 kDa.
Related Product 1: Yes-pY222+pY223 blocking peptide
Scientific Background: Yes (Yes1) is a protein-tyrosine kinase of the TK group and Src family. It promotes G2/M cell cycle progression and cytokinesis. Y426F mutation in Yes leads to about half loss of CSK-mediated inhibition. Yes may be an oncoprotein (OP) based on its similarity to other Src family protein-tyrosine kinases. It is the cellular homologue of the Yamaguchi sarcoma virus oncoprotein. By isotopic in situ hybridization, Yes1 gene localization is frequently associated with chromosome translocation and pathogenesis of follicular lymphoma.
