Product Name:
Yes-pY222+pY223
Product Number:
ab-pk858
Target Full Name: Yamaguchi sarcoma proto-oncogene-encoded tyrosine kinase
Target Alias: c-Yes; HsT441; p61-Yes; CCDS11824.1; ENSG00000176105
Product Type Specific: Yes protein kinase phosphosite-specific antibody
Antibody Code: PK858
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y222+Y223
Protein UniProt: P07947
Protein SigNET: Yes
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Yes sequence peptide Cat. No.: PE-04AHY99
Antibody Immunogen Sequence: NGG(pY)(pY)ITT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N219 to T226; in the SH2 domain. The effects of Y222 and Y223 phosphorylation are unclear. These are two of the major in vivo phosphorylation sites in Yes (Y222 has >1816 reports; Y223 has > 675 reports) in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-65 kDa.
Related Product 1: Yes (219-226) pY222+pY223 antibody blocking peptide (Cat. No.: PE-04AHY99)
Scientific Background: Yes (Yes1) is a protein-tyrosine kinase of the TK group and Src family. It is a cellular homolog of the Yamaguchi sarcoma virus oncoprotein that regulates crucial cellular processes, including cell adhesion, growth, migration, and cytoskeleton remodeling, particularly at cell-cell junctions. It promotes G2/M cell cycle progression and cytokinesis. It is important for maintaining vascular barrier integrity, controlling endothelial cell migration, and regulating cell-cell junctions. It also plays a key role in mediating T-cell migration. Upon stimulation by receptor tyrosine kinases (e.g., EGFR, PDGFR), Yes is recruited and activated, subsequently phosphorylating downstream substrates to promote cell survival. It can be inactivated by the kinase Csk. Y426F mutation in Yes leads to about half loss of CSK-mediated inhibition. Yes may be an oncoprotein (OP) based on its similarity to other Src family protein-tyrosine kinases. By isotopic in situ hybridization, Yes1 gene localization is frequently associated with chromosome translocation and pathogenesis of follicular lymphoma. The protein is often highly expressed in cancers such as esophageal, lung, and bladder cancer. This description may include information annotated by UniProt and/or Google AI.