Product Name:
Wee1-pS642
Product Number:
ab-pk854
Target Full Name: Wee1 protein-tyrosine kinase
Target Alias: Kinase WEE1; WEE1; WEE1A; WEE1hu
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK854
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S642
Protein UniProt: P30291
Protein SigNET: P30291
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Wee1 sequence peptide Cat. No.: PE-04AQN99
Antibody Immunogen Sequence: RSV(pS)LTI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R639 to I645; In the C-terminus of the kinase
Production Method: Corresponds to amino acid residues R639 to I645; In the C-terminus of the kinase
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in sea star oocytes. Very weak detection of target in MCF7 and T98G cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-74 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells, except for a 25 kDa protein; phenylarsine oxide (PAO) increases strong 50 kDa cross-reactive protein in HeLa cells. The 25 kDa cross-reactive protein was also detected in sea star oocytes and MCF7 and T98G cells.
Related Product 1: Wee1-pS642 blocking peptide
Scientific Background: Wee1 is a Dual specificity protein kinase of the Other group and WEE family. It is a negative regulator to entry into G2-M transition in mitosis through phosphorylation of CDK1. Wee1 is a nuclear protein that catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. Phosphorylatio of S121 induces proteolysis through interaction with BTRC and FBW1B. Phosphorylation of S53 and S123 beta-transducin repeat-containing protein recognition for degradation by ubiquitination. Phosphorylation of T187, T190 and S193 induces proteolysis through interaction with BTRC and FBW1B. Phosphorylation of S642 inhibits phosphotransferase activity and induces interaction with 14-3-3 theta. Wee1 is highly expressed in testis. Wee1 kinase phosphorylates the p34 (CDC2) -cyclin B complex on Y15 and inactivates the p34 (CDC2)-cyclin B kinase. Wee1 is a target for cancer therapy. Specific inhibition of Wee1 can be used to increase the sensitivity of T-cell leukemia and pancreatic cancer. Wee1 was found to be a novel independent prognostic marker of poor survival ovarian carcinomas.
